MicroRNAs control eyelid development through regulating Wnt signaling
| Autor: | Qiliang Zhou, Mitsue Hishinuma, Atsushi Kitatmura, Akihiro Yasue, Akane Yamada, Takahiro Nagai, Maiko Kawasaki, Paul T. Sharpe, Fumiya Meguro, Yurie Yamada, Yoko Otsuka-Tanaka, Yasuo Saijo, Atsushi Ohazama, Yasumitsu Kodama, Katsushige Kawasaki, Takeyasu Maeda, James Blackburn, Robert Hindges, Ritsuo Takagi, Supaluk Trakanant, Momoko Watanabe |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Ribonuclease III Organogenesis Mutant Morphogenesis mesenchyme Biology Fibroblast growth factor Shh DEAD-box RNA Helicases 03 medical and health sciences Wnt Mice 0302 clinical medicine microRNA Gene expression Bmp Fgf Animals Wnt Signaling Pathway dicer Wnt signaling pathway Eyelids Gene Expression Regulation Developmental Phenotype Cell biology MicroRNAs 030104 developmental biology biology.protein 030217 neurology & neurosurgery eyelid development Developmental Biology Dicer |
| Zdroj: | Nagai, T, Trakanant, S, Kawasaki, M, Kawasaki, K, Yamada, Y, Watanabe, M, Blackburn, J, Otsuka-Tanaka, Y, Hishinuma, M, Kitatmura, A, Meguro, F, Yamada, A, Kodama, Y, Maeda, T, Zhou, Q, Saijo, Y, Yasue, A, Sharpe, P T, Hindges, R, Takagi, R & Ohazama, A 2019, ' MicroRNAs control eyelid development through regulating Wnt signaling ', Developmental Dynamics, vol. 248, no. 3, pp. 201-210 . https://doi.org/10.1002/dvdy.10 |
| ISSN: | 1097-0177 |
| Popis: | Background The timing, location, and level of gene expression are crucial for normal organ development, because morphogenesis requires strict genetic control. MicroRNAs (miRNAs) are noncoding small single-stranded RNAs that play a critical role in regulating gene expression level. Although miRNAs are known to be involved in many biological events, the role of miRNAs in organogenesis is not fully understood. Mammalian eyelids fuse and separate during development and growth. In mice, failure of this process results in the eye-open at birth (EOB) phenotype. Results It has been shown that conditional deletion of mesenchymal Dicer (an essential protein for miRNA processing; Dicer fl/fl ;Wnt1Cre) leads to the EOB phenotype with full penetrance. Here, we identified that the up-regulation of Wnt signaling resulted in the EOB phenotype in Dicer mutants. Down-regulation of Fgf signaling observed in Dicer mutants was caused by an inverse relationship between Fgf and Wnt signaling. Shh and Bmp signaling were down-regulated as the secondary effects in Dicer fl/fl ;Wnt1Cre mice. Wnt, Shh, and Fgf signaling were also found to mediate the epithelial-mesenchymal interactions in eyelid development. Conclusions miRNAs control eyelid development through Wnt. Developmental Dynamics 248:201-210, 2019. © 2019 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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