Derivatization Strategies for the Detection of Triamcinolone Acetonide in Cartilage by Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
| Autor: | Barré, Florian P. Y., Flinders, Bryn, Garcia, João P., Jansen, Imke, Huizing, Lennart R. S., Porta, Tiffany, Creemers, Laura B., Heeren, Ron M. A., Cillero-Pastor, Berta |
|---|---|
| Přispěvatelé: | Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS) |
| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Triamcinolone acetonide Electrospray ionization Anti-Inflammatory Agents Cartilage metabolism Osteoarthritis Mass spectrometry Triamcinolone Acetonide 01 natural sciences Mass spectrometry imaging Analytical Chemistry 03 medical and health sciences Journal Article medicine Humans Chromatography Chemistry Cartilage 010401 analytical chemistry medicine.disease digestive system diseases 3. Good health 0104 chemical sciences Betaine Matrix-assisted laser desorption/ionization 030104 developmental biology medicine.anatomical_structure Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine.drug |
| Zdroj: | Analytical Chemistry, 88(24), 12051. American Chemical Society Analytical Chemistry Analytical Chemistry, 88(24), 12051-12059. American Chemical Society |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b02491 |
| Popis: | Osteoarthritis (OA), characterized by degeneration of the cartilaginous tissue in articular joints, severely impairs mobility in many people worldwide. The degeneration is thought to be mediated by inflammatory processes occurring in the tissue of the joint, including the cartilage. Intra-articular administered triamcinolone acetonide (TAA) is one of the drug treatments employed to ameliorate the inflammation and pain that characterizes OA. However, the penetration and distribution of TAA into the avascular cartilage is not well understood. We employed matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which has been previously used to directly monitor the distribution of drugs in biological tissues, to evaluate the distribution of TAA in human cartilage after in vitro incubation. Unfortunately, TAA is not easily ionized by regular electrospray ionization (ESI) or MALDI. To overcome this problem, we developed an on-tissue derivatization method with Girard's reagent T (GirT) in human incubated cartilage being able to study its distribution and quantify the drug abundance (up to 3.3 ng/mu L). Our results demonstrate the depth of penetration of a corticosteroid drug in human OA cartilage using MALDI-MSI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|