Growth of Anaerobic Methane-Oxidizing Archaea and Sulfate-Reducing Bacteria in a High-Pressure Membrane Capsule Bioreactor

Autor: Peer H. A. Timmers, Alfons J. M. Stams, Roel J.W. Meulepas, Piet N.L. Lens, Jarno Gieteling, Caroline M. Plugge, H. C. Aura Widjaja-Greefkes
Přispěvatelé: Universidade do Minho
Rok vydání: 2015
Předmět:
Geologic Sediments
Applied Microbiology and Biotechnology
Methane
chemistry.chemical_compound
Bioreactors
Microbiologie
Environmental Microbiology
cold-seep sediments
Sulfate-reducing bacteria
Phylogeny
chemistry.chemical_classification
0303 health sciences
Ecology
biology
16s ribosomal-rna
Sulfates
hydrothermal sediments
marine-sediments
gradient gel-electrophoresis
Environmental chemistry
Milieutechnologie
community structure
guaymas basin
Oxidation-Reduction
Biotechnology
Sulfide
Methanogenesis
Molecular Sequence Data
Microbiology
03 medical and health sciences
Pressure
Bioreactor
Ecosystem
030304 developmental biology
WIMEK
Science & Technology
Sulfur-Reducing Bacteria
030306 microbiology
population-dynamics
biology.organism_classification
Archaea
chemistry
13. Climate action
microbial diversity
Anaerobic oxidation of methane
Environmental Technology
gene database
Desulfuromonadales
Bacteria
Food Science
Zdroj: Applied and Environmental Microbiology 81 (2015) 4
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Applied and Environmental Microbiology, 81(4), 1286-1296
ISSN: 1098-5336
0099-2240
Popis: Communities of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria (SRB) grow slowly, which limits the ability to perform physiological studies. High methane partial pressure was previously successfully applied to stimulate growth, but it is not clear how different ANME subtypes and associated SRB are affected by it. Here, we report on the growth of ANME-SRB in a membrane capsule bioreactor inoculated with Eckernförde Bay sediment that combines high-pressure incubation (10.1 MPa methane) and thorough mixing (100 rpm) with complete cell retention by a 0.2-m-pore-size membrane. The results were compared to previously obtained data from an ambient-pressure (0.101 MPa methane) bioreactor inoculated with the same sediment. The rates of oxidation of labeled methane were not higher at 10.1 MPa, likely because measurements were done at ambient pressure. The subtype ANME-2a/b was abundant in both reactors, but subtype ANME-2c was enriched only at 10.1 MPa. SRB at 10.1 MPa mainly belonged to the SEEP-SRB2 and Eel-1 groups and the Desulfuromonadales and not to the typically found SEEP-SRB1 group. The increase of ANME-2a/b occurred in parallel with the increase of SEEP-SRB2, which was previously found to be associated only with ANME-2c. Our results imply that the syntrophic association is flexible and that methane pressure and sulfide concentration influence the growth of different ANME-SRB consortia. We also studied the effect of elevated methane pressure on methane production and oxidation by a mixture of methanogenic and sulfate-reducing sludge. Here, methane oxidation rates decreased and were not coupled to sulfide production, indicating trace methane oxidation during net methanogenesis and not anaerobic methane oxidation, even at a high methane partial pressure.
This work was supported in part by the EET program of the Dutch Ministries of Economic Affairs; Education, Culture and Science; and Environment and special planning through the Anaerobic Methane Oxidation for Sulfate Reduction project. This research was also supported by the Dutch Technology Foundation STW, which is part of the Netherlands Organization for Scientific Research (NWO) and which is partly funded by the Ministry of Economic Affairs. The research of A.J.M.S. is supported by an ERC grant (project 323009) and a Gravitation grant (project 024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Science Foundation (NWO).
Databáze: OpenAIRE
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