NtTPN1: a RPP8-like R gene required for Potato virus Y-induced veinal necrosis in tobacco

Autor: François Dorlhac de Borne, Véronique Decroocq, Emilie Julio, Thierry Candresse, Laurent Glais, Benoît Moury, R. Volpatti, J. Cotucheau, Vincent Michel, Sylvie German-Retana, C. Decorps
Přispěvatelé: Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Imperial Tobacco Group, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Fédération Nationale des Producteurs de Plants de Pomme de Terre (FN3PT), ANR-10-INBS-04, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), German-Retana, Sylvie, Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Hypersensitive response
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Phytopathology and phytopharmacy
Nicotiana tabacum
Virologie
Potyvirus
Locus (genetics)
Plant Science
01 natural sciences
PVY
resistance
03 medical and health sciences
veinal necrosis
Plant virus
Virology
santé des plantes
HR
Genetics
Allele
R gene
pathologie végétale
RPP8-like gene
tolerance
résistance variétale
virus phytopathogène
biology
Cell Biology
biology.organism_classification
gène de résistance
Phytopathologie et phytopharmacie
virus y de la pomme de terre
Agricultural sciences
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
tabac
030104 developmental biology
Potato virus Y
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Sciences agricoles
010606 plant biology & botany
Zdroj: Plant Journal
Plant Journal, Wiley, 2018, 95 (4), pp.700-714. ⟨10.1111/tpj.13980⟩
Plant Journal, 2018, 95 (4), pp.700-714. ⟨10.1111/tpj.13980⟩
Plant Journal 4 (95), 700-714. (2018)
ISSN: 1365-313X
0960-7412
DOI: 10.1111/tpj.13980⟩
Popis: UMR BFP - Equipe Virologie; International audience; Potato virus Y is one of the most damaging viruses of tobacco. In particular, aggressive necrotic strains (PVYN) lead to considerable losses in yield. The main source of resistance against PVY is linked to the va locus. However, va‐overcoming PVY isolates inducing necrotic symptoms were observed in several countries. In this context, it is important to find va‐independent protection strategies. In a previous study, the phenotyping of 162 tobacco varieties revealed ten accessions that do not carry the va allele and do not exhibit typical PVYN‐induced veinal necrosis. Despite the absence of necrotic symptoms, normal viral accumulation in these plants suggests a va‐independent mechanism of tolerance to PVYN‐induced systemic veinal necrosis. Fine mapping of the genetic determinant(s) was performed in a segregating F2 population. The tolerance trait is inherited as a single recessive gene and allelism tests demonstrated that eight of the ten tolerant varieties carry the same determinant. Anchoring the linkage map to the tobacco genome physical map allowed the identification of a RPP8‐like R gene, called NtTPN1 (for Nicotiana tabacum Tolerance to PVY‐induced Necrosis1), with the same single‐nucleotide polymorphism in the eight tolerant accessions. Functional assays using homozygous NtTPN1 EMS mutants confirmed the role of NtTPN1 in the tolerance phenotype. PVYN‐induced systemic veinal necrosis in tobacco likely represents an inefficient defense response with HR‐like characteristics. The identification of NtTPN1 opens breeding options to minimize the impact of emerging and so far uncontrolled va‐breaking necrotic PVY isolates.
Databáze: OpenAIRE
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