Barcoding chrysomelidae: a resource for taxonomy and biodiversity conservation in the Mediterranean Region
| Autor: | Mostafa Mirzaei, Matteo Montagna, Giuseppe Carlo Lozzia, Carlo Leonardi, Mauro Daccordi, Davide Sassi, Giulia Magoga, Renato Regalin |
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| Přispěvatelé: | Giulia, Magoga, Sassi, D., Daccordi, M., Leonardi, C., Mirzaei, M., Regalin, R., Lozzia, G., Montagna, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecta Arthropoda Leaf beetles Chrysomeloidea Biodiversity Biology C-bar project 010603 evolutionary biology 01 natural sciences DNA barcoding Molecular taxonomy 03 medical and health sciences Pachybrachis Animalia Leaf beetle Exosoma lusitanicum Endemism Ecology Evolution Behavior and Systematics Chrysomelidae Ecology biology.organism_classification Coleoptera Cytochrome c oxidase subunit 1 030104 developmental biology Animal Science and Zoology Taxonomy (biology) Cryptocephalus Research Article |
| Zdroj: | ZooKeys ZooKeys 597: 27-38 |
| Popis: | The Mediterranean Region is one of the world’s biodiversity hot-spots, which is also characterized by high level of endemism. Approximately 2100 species of leaf beetle (Coleoptera; Chrysomelidae) are known from this area, a number that increases year after year and represents 5/6% of the known species. These features, associated with the urgent need to develop a DNA-based species identification approach for a broad spectrum of leaf beetle species, prompted us to develop a database of nucleotide sequences, with a solid taxonomic background, for all the Chrysomelidae Latreille, 1802 sensu latu inhabiting the Mediterranean region. The Mediterranean Chrysomelidae Barcoding project, which has started in 2009, involves more than fifty entomologists and molecular biologists from different European countries. Numerous collecting campaigns have been organized during the first seven years of the project, which led to the collection of more than 5000 leaf beetle specimens. In addition, during these collecting campaigns two new allochthonous species for Europe, namely Ophraella communa LeSage, 1986 and Colasposoma dauricum Mannerheim, 1849, were intercepted and some species new to science were discovered (e.g., Pachybrachis sassii Montagna, 2011 and Pachybrachis holerorum Montagna et al., 2013). DNA was extracted from 1006 specimens (~13% of the species inhabiting the Mediterranean region) and a total of 910 cox1 gene sequences were obtained (PCR amplification efficiency of 93.8%). Here we report the list of the barcoded subfamilies, genera and the number of species for which cox1 gene sequences were obtained; the metadata associated with each specimen and a list of problematic species for which marker amplification failed. In addition, the nucleotide divergence within and between species and genera was estimated and values of intraspecific nucleotide divergence greater than the average have been discussed. Cryptocephalus quadripunctatus G. A. Olivier, 1808, Cryptocephalus rugicollis G. A. Olivier, 1791 and Exosoma lusitanicum Linnaeus, 1767) are representatives of these cases. |
| Databáze: | OpenAIRE |
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