Macrophage prostaglandin E2 and oxidative responses to endotoxin during immunosuppression associated with anaesthesia and transfusion
| Autor: | P.L. Yap, D.C. Carter, Robin J Prescott, Jian-Hua Mao, G.M. Raab, B. H. Su, W.B. Ross, W. Qian, H.A. Leaver |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Blood transfusion medicine.medical_treatment Clinical Biochemistry Stimulation Oxidative phosphorylation Dinoprostone Superoxides Immune Tolerance Animals Medicine Anesthesia Prostaglandin E2 Dose-Response Relationship Drug business.industry Transfusion Reaction Immunosuppression Cell Biology Rats Endotoxins Eicosanoid Rats Inbred Lew Immunology Macrophages Peritoneal lipids (amino acids peptides and proteins) business Oxidation-Reduction Intracellular Prostaglandin E medicine.drug |
| Zdroj: | Prostaglandins, Leukotrienes and Essential Fatty Acids. 49:945-953 |
| ISSN: | 0952-3278 |
| Popis: | The widespread use of blood transfusion in major surgical procedures has led to concern about the immunosuppressive effect of transfusion on patients with underlying malignancy. Transfusion may also suppress the host response to infection. The cellular mechanisms of transfusion-associated immunosuppression may involve macrophage prostaglandin E 2 (PGE 2 ) in modulating the host response to cancer and infection. We previously observed that the transfusion of blood increased PGE 2 production by unstimulated macrophages. To investigate this PGE 2 associated immunosuppression, we studied the effect of transfusion of rats using a physiological stimulus of macrophage PGE 2 production, bacterial endotoxin. In the same macrophages, we analysed intracellular oxidative activity. Both allogeneic and syngeneic blood transfusion were associated with increased PGE 2 release by macrophages. This stimulation of PGE 2 increased with duration of storage of blood. A similar effect of serum indicated that a humoral factor was involved. Endotoxin (50 ng/ml–500 μg/ml) stimulated PGE 2 production in all transfused subjects. The lowest endotoxin concentration gave proportionately the greatest stimulation. Oxidative activity was down-regulated in macrophages of transfused rats, supporting an immunosuppressive role of PGE 2 within the macrophage. An effect of surgery on the oxidative response was also detected. |
| Databáze: | OpenAIRE |
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