Quantification of isoflavones and lignans in serum using isotope dilution liquid chromatography/tandem mass spectrometry
| Autor: | Sheila Bingham, Philip B. Grace, Tara Fryatt, Mark F. Oldfield, James I. Taylor, Nigel P. Botting, Nawaf Al-Maharik |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Genistein Phytoestrogens Sensitivity and Specificity Lignans Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Isotopes Liquid chromatography–mass spectrometry Humans Estrogens Non-Steroidal Enterodiol Spectroscopy Chromatography Organic Chemistry Daidzein Reproducibility of Results food and beverages Glycitein Equol Isoflavones United Kingdom chemistry Calibration Female Plant Preparations Chromatography Liquid |
| Zdroj: | Rapid Communications in Mass Spectrometry. 17:1350-1357 |
| ISSN: | 1097-0231 0951-4198 |
| Popis: | Phytoestrogens (isoflavones and lignans) are receiving increasing attention due to a potential protective effect against a number of complex diseases. However, in order to investigate these associations, it is necessary to accurately quantify the levels of phytoestrogens in foods and biological fluids. We report an assay for three isoflavones (daidzein, genistein, and glycitein), two metabolites of daidzein (O-desmethylangolensin and equol), and two lignans (enterodiol and enterolactone) in human serum using electrospray ionisation liquid chromatography/mass spectrometry (LC/MS) with selective reaction monitoring. A simple, highly automated sample preparation procedure requires only 200 microL of sample and utilises one solid-phase extraction stage. Limits of detection are in the region of 10 pg/mL for all analytes except equol, which had a limit of detection of approximately 100 pg/mL. The method developed is suitable for measuring the concentrations of phytoestrogens in blood samples collected from large epidemiological studies. The results of the analysis of serum samples from 300 men and women living in the UK are reported. |
| Databáze: | OpenAIRE |
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