Immunohistochemical analysis of presenilin-1 expression in the mouse brain
| Autor: | M. Gohin, B. Bonici, Laurent Pradier, Saliha Moussaoui, Frédéric Revah, Assunta Imperato, Véronique Blanchard, Christian Czech |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Amyloid Transcription Genetic Neurite Molecular Sequence Data Biophysics Gene Expression Neurodegenerative disease Biochemistry Presenilin Mice Alzheimer Disease Structural Biology Presenilin-1 Genetics Animals Humans Amino Acid Sequence RNA Messenger Molecular Biology Gene Chromosomes Human Pair 14 Antiserum Mice Inbred BALB C Mouse brain biology Brain Membrane Proteins Chromosome Cell Biology Alzheimer's disease Chromosome 14 Immunohistochemistry Molecular biology Peptide Fragments Polyclonal antibodies Protein Biosynthesis biology.protein Immunostaining |
| Zdroj: | FEBS Letters. 383:219-222 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(96)00250-5 |
| Popis: | At least 22 different mutations associated with early-onset familial Alzheimer's disease (AD) in various kindreds have been reported to occur in a recently identified gene on chromosome 14, presenilin 1 (PS-1) (Sherrington et al. (1995) Nature 375, 754–760 [1] and reviewed by Van Broeckhoven (1995) Nat. Genet. 11, 230–231 [2]). In order to study the localization of PS-1 in the brain, we raised a polyclonal antiserum specific to a fragment of the predicted protein sequence of PS-1. PS-1 immunostaining was found intracellularly, in the perikaria of discrete cells, mostly neurons, appearing as thick granules, resembling large-size vesicles. These granules were located in the periphery of cell bodies and extended into dendrites and neurites. PS-1 expression was found to be broadly distributed throughout the mouse brain, not only in structures involved in AD pathology, but also in structures unaltered by this disease. |
| Databáze: | OpenAIRE |
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