Novel Description of mcl-PHA Biosynthesis by Pseudomonas chlororaphis from Animal-Derived Waste
| Autor: | Sigurd Schober, Angelika Reiterer, Martin Koller, Eva Maria Rechberger, Martin Mittelbach, Alexander Muhr, Katharina Strohmeier, Karin Malli, Anna Salerno |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Kinetic analysis
PHA biosynthesis Bioengineering Applied Microbiology and Biotechnology Polyhydroxyalkanoates Microbiology Fats 03 medical and health sciences Tallow Pseudomonas Food science 030304 developmental biology 0303 health sciences Biodiesel biology 030306 microbiology Chemistry Product recovery General Medicine Pseudomonas chlororaphis biology.organism_classification 6. Clean water Kinetics Biofuels Fermentation Caprylates Biotechnology |
| Zdroj: | Journal of Biotechnology |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2013.02.003 |
| Popis: | A novel description of mcl -PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (μ max. ) of 0.08 h −1 , 0.10 h −1 and 0.13 h −1 , respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl -PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum. |
| Databáze: | OpenAIRE |
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