Identification of Membrane Calcium Channels Essential for Cytoplasmic and Nuclear Calcium Elevations Induced by Vascular Endothelial Growth Factor in Human Endothelial Cells
| Autor: | Stéphanie Garnier-Raveaud, Michel Robert-Nicoud, Yves Usson, Francine Cand, Gilles Faury, Jean Verdetti |
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| Přispěvatelé: | RFMQ, Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Usson, Yves, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2001 |
| Předmět: |
Vascular Endothelial Growth Factor A
Cytoplasm Umbilical Veins Patch-Clamp Techniques [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Angiogenesis Clinical Biochemistry Endothelial Growth Factors MESH: Vascular Endothelial Growth Factors Calcium in biology MESH: Endothelial Growth Factors 0302 clinical medicine Endocrinology Nickel MESH: Nickel MESH: Microscopy Confocal Cells Cultured Lymphokines 0303 health sciences MESH: Electrophysiology Microscopy Confocal Voltage-dependent calcium channel Vascular Endothelial Growth Factors MESH: Infant Newborn Cell biology Electrophysiology Vascular endothelial growth factor B Vascular endothelial growth factor A MESH: Calcium 030220 oncology & carcinogenesis MESH: Calcium Channels MESH: Cell Division MESH: Endothelium Vascular Cell Division MESH: Cells Cultured MESH: Cell Nucleus Transcriptional Activation Inositol Phosphates Recombinant Fusion Proteins MESH: Umbilical Veins chemistry.chemical_element Calcium Biology 03 medical and health sciences MESH: Patch-Clamp Techniques MESH: Recombinant Fusion Proteins Humans 030304 developmental biology Cell Nucleus MESH: Humans MESH: Lymphokines MESH: Vascular Endothelial Growth Factor A MESH: Cytoplasm Calcium channel Cell Membrane Infant Newborn T-type calcium channel Cell Biology MESH: Inositol Phosphates [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging chemistry MESH: Trans-Activation (Genetics) Calcium Channels Endothelium Vascular MESH: Cell Membrane |
| Zdroj: | Growth Factors Growth Factors, Taylor & Francis, 2001, 19 (1), pp.35-48 |
| ISSN: | 1029-2292 0897-7194 |
| Popis: | Vascular endothelial growth factor (VEGF) is mitogenic for endothelial cells and has been shown to induce angiogenesis and endothelial cell migration through stimulation of endothelial tyrosine-kinase receptors. Here, using confocal microscopy and the patch-clamp technique on endothelial cells, membrane permeability to calcium as well as cytoplasmic and nuclear free calcium levels have been investigated in the first stages of tyrosine-kinase receptor activation by VEGF. VEGF (0.5nM) as well as inositol trisphosphate (IP3) induced an activation of membrane calcium-permeable channels exhibiting a similar low conductance in the range of 10 pS. The VEGF-triggered activation of these calcium channels, mediated by IP3 and involving the intracellular calcium stores, results in an increase in both cytoplasmic and nuclear calcium levels in endothelial cells, potentially modulating gene expression. Finally, the effect of Ni2+, a calcium channel blocker, on endothelial cell proliferation has been studied. The results show that inhibition of extracellular calcium influx significantly inhibits VEGF-induced cell proliferation. In the process of cell stimulation by VEGF, and possibly by other growth factors, activation of calcium channels could then be a key step in calcium-regulated gene expression and cell activation. These results suggest that the use of calcium channel blockers could be a novel way of prevention or reversion of VEGF-induced tumoral angiogenesis. |
| Databáze: | OpenAIRE |
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