Visualization of Lipid Components in Human Coronary Plaques Using Color Fluorescence Angioscopy
| Autor: | Yasuto Uchida, Yasumi Uchida, Noriaki Kameda |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Apolipoprotein B
Color Angioscopy Coronary Artery Disease HOMIDIUM BROMIDE chemistry.chemical_compound medicine Humans Apolipoproteins B medicine.diagnostic_test biology Lysophosphatidylcholines General Medicine Atherosclerosis Lipids Molecular biology Fluorescence Plaque Atherosclerotic Lipoproteins LDL Autofluorescence Lysophosphatidylcholine Microscopy Fluorescence chemistry biology.protein lipids (amino acids peptides and proteins) Trypan blue Cardiology and Cardiovascular Medicine Lipoprotein |
| Zdroj: | Circulation Journal. 74:2181-2186 |
| ISSN: | 1347-4820 1346-9843 |
| DOI: | 10.1253/circj.cj-10-0451 |
| Popis: | Background: If oxidized low-density lipoprotein (oxLDL), LDL, lysophosphatidylcholine (LPC) and apolipoprotein B (apoB) can be visualized simultaneously, their roles in the initiation, progression and destabilization of atherosclerotic plaques can be objectively evaluated. Methods and Results: (1) The fluorescence characteristic of each atherogenic substance was investigated by microscopy using a band-pass filter (470 nm) and a band-absorption filter (520 nm) with homidium bromide (Ho) and trypan blue (TB) as indicators. (2) 50 excised human coronary plaques were classified by their autofluorescence into green, greenish-yellow and yellow, and the localization of oxLDL, LDL, LPC and apoB were investigated by color fluorescence angioscopy (CFA). The plaque colors were white, yellow and glistening yellow by conventional angioscopy. (1) OxLDL and LDL exhibited golden fluorescence, whereas LPC and apoB exhibited red fluorescence. (2) By CFA, 16 of 19 greenish-yellow and 1 of 8 yellow plaques exhibited red and golden fluorescence in a mosaic pattern, indicating co-deposition of oxLDL/LDL and LPC/apoB; 3 greenish-yellow and 7 yellow plaques exhibited red fluorescence, indicating solitary deposition of apoB; 23 green plaques infrequently exhibited these fluorescence colors. Conclusions: OxLDL/LDL and LPC/apoB were successfully visualized as co-deposited in greenish-yellow autofluorescence plaques, but only LPC/apoB in yellow autofluorescence plaques. (Circ J 2010; 74: 2181-2186) |
| Databáze: | OpenAIRE |
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