Bilateral canine seminoma with ocular metastasis: histochemical and immunohistochemical characterization
| Autor: | Germana Alegro da Silva, Noeme Sousa Rocha, Renata S. Belluci, Cláudia Valéria Seullner Brandão, Paula de Sanctis, Luis Gabriel Rivera-Calderón, Luis Mauricio Montoya-Flórez |
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| Přispěvatelé: | Universidad Antonio Nariño (UAN), Universidad de Caldas, Universidade Estadual Paulista (Unesp), GIDIMEVETZ Universidad Pedagógica y Tecnológica de Colombia |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Retina Conjunctiva Neoplasia business.industry Veterinary (miscellaneous) Enucleation Seminoma medicine.disease Immunohistochemistry Metastasis C-KIT chemistry.chemical_compound medicine.anatomical_structure Castration chemistry Dog medicine Ki-67 business Inhibin-α |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1983-0246 |
| DOI: | 10.24070/bjvp.1983-0246.v13i1p57-61 |
| Popis: | Made available in DSpace on 2020-12-12T02:22:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-01 A 10-year old male dog was examined due to a buphthalmia in the left eye and a nodule in the two testicles. Due to the limited resources of the owner and loss of visual acuity of the patient, the enucleation and castration were chosen as treatment. Microscopic analysis of the testicular tissue revealed neoplastic germ cells. Morphologically, neoplastic cells were characterized by distinct cell borders, scarce and eosinophilic cytoplasm, large round nucleus, with thick chromatin and a prominent nucleolus. Binucleated and multinucleated neoplastic cells were also frequently observed. In 10 high power fields (400x), 62 typical and atypical mitosis were counted. Similar neoplastic cells were identified within the vessels of the retina, sclera and in the sub-epithelial conjunctive tissue of the eyelid. The neoplastic cells observed in the testicle and in the eye were positive for PAS. By immunochemistry technique was identified an intense immunostaining of the neoplastic cells for Vimentin and Ki-67 in both testicular and ocular tissue. While, discrete immunoreactivity was identified to c-KIT from the neoplastic cells in both organs. Based on morphological, histochemical and immunohistochemical analysis, it was possible to characterize the ocular lesion as seminoma metastasis. College of Veterinary Medicine and Animal Husbandry Universidad Antonio Nariño (UAN) Faculty of Veterinary Medicine Universidad Nacional de Colombia Veterinary Pathology Research Group Universidad de Caldas Department of Veterinary Clinic and Surgery College of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) Pathology College of Medicine from Botucatu São Paulo State University Department of Veterinary Clinic College of Veterinary Medicine and Animal Husbandry São Paulo State University GIDIMEVETZ Universidad Pedagógica y Tecnológica de Colombia Department of Veterinary Clinic and Surgery College of Agricultural and Veterinarian Sciences São Paulo State University (UNESP) Pathology College of Medicine from Botucatu São Paulo State University Department of Veterinary Clinic College of Veterinary Medicine and Animal Husbandry São Paulo State University |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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