Towards chemiluminescence detection in micro-sequential injection lab-on-valve format: A proof of concept based on the reaction between Fe(II) and luminol in seawater

Autor: Jaromir Ruzicka, Hugo M. Oliveira, Maxime M. Grand, Christopher I. Measures
Rok vydání: 2015
Předmět:
Zdroj: Talanta. 133:107-111
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2014.06.076
Popis: Micro-sequential injection lab-on-valve (µSI-LOV) is a well-established analytical platform for absorbance and fluorescence based assays but its applicability to chemiluminescence detection remains largely unexplored. In this work, we describe a novel fluidic protocol and two distinct strategies for photon collection that enable chemiluminescence detection using µSI-LOV for the first time. To illustrate this proof of concept, we selected the reaction between Fe(II) and luminol and developed a preliminary protocol for Fe(II) determinations in acidified seawater. The optimized fluidic strategy consists of holding 100 µL of the luminol reagent in a confined zone of the LOV and then displacing it with 50 µL of sample while monitoring the chemiluminescent product. Detection is achieved using two strategies: one based on a bifurcated optical fiber and the other based on a customized detection window created by mounting a photomultiplier tube atop of the LOV device. We show that detection is possible using both strategies but that the window strategy yields significantly enhanced sensitivity (355×) due to the larger detection area. In our final experimental conditions and using window detection, it was possible to achieve a limit of detection (LOD) of 1 nmol L(-1) and to quantify Fe(II) in acidified seawater samples up to 20.00 nmol L(-1) with high precision (RSD
Databáze: OpenAIRE
Externí odkaz: