Development of a Polyphenol Oxidase Biosensor from Jenipapo Fruit Extract (Genipa americana L.) and Determination of Phenolic Compounds in Textile Industrial Effluents
| Autor: | Douglas Vieira Thomaz, Flavio Marques Lopes, Rafael Luque, Germán Sanz Lobón, Denes Ferraz, Luane Ferreira Garcia, Eric de Souza Gil, Rafael Souza Antunes |
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| Rok vydání: | 2018 |
| Předmět: |
biosensor enzyme
Genipa americana L polifenoloxidases phenolic compounds Calibration curve lcsh:Biotechnology Clinical Biochemistry Rubiaceae 02 engineering and technology 01 natural sciences Polyphenol oxidase Article chemistry.chemical_compound Phenols lcsh:TP248.13-248.65 Genipa americana Effluent Detection limit Catechol Chromatography biology Chemistry Plant Extracts Textiles 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Biosensor enzyme Phenolic compounds 0104 chemical sciences Fruit Polifenoloxidases Differential pulse voltammetry 0210 nano-technology Biosensor Catechol Oxidase |
| Zdroj: | Biosensors 8, 47 (2018) Helvia. Repositorio Institucional de la Universidad de Córdoba instname Biosensors Biosensors, Vol 8, Iss 2, p 47 (2018) Biosensors; Volume 8; Issue 2; Pages: 47 |
| Popis: | In this work, an innovative polyphenol oxidase biosensor was developed from Jenipapo (Genipa americana L.) fruit and used to assess phenolic compounds in industrial effluent samples obtained from a textile industry located in Jaraguá-GO, Brasil. The biosensor was prepared and optimized according to: the proportion of crude vegetal extract, pH and overall voltammetric parameters for differential pulse voltammetry. The calibration curve presented a linear interval from 10 to 310 M (r2 = 0.9982) and a limit of detection of 7 M. Biosensor stability was evaluated throughout 15 days, and it exhibited 88.22% of the initial response. The amount of catechol standard recovered post analysis varied between 87.50% and 96.00%. Moreover, the biosensor was able to detect phenolic compounds in a real sample, and the results were in accordance with standard spectrophotometric assays. Therefore, the innovatively-designed biosensor hereby proposed is a promising tool for phenolic compound detection and quantification when environmental contaminants are concerned |
| Databáze: | OpenAIRE |
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