Multi-Biochemical Test System for Distinguishing Enteric and Other Gram-Negative Bacilli
| Autor: | Joann P. Stanek, Judith A. Baudo, Mercedes Schaab, Harry R. Elston |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1971 |
| Předmět: |
Salmonella
Bacilli food.ingredient Indoles Carboxy-Lyases medicine.disease_cause General Biochemistry Genetics and Molecular Biology Microbiology chemistry.chemical_compound food Species Specificity Escherichia medicine Methods Agar Shigella Hydrogen Sulfide General Pharmacology Toxicology and Pharmaceutics Lactose Bacteriological Techniques General Immunology and Microbiology biology Lysine decarboxylase Bacteria Lysine Phenylalanine Hydroxylase General Medicine biology.organism_classification Urease Culture Media chemistry Clinical Microbiology Virology and Immunology |
| Popis: | A multi-biochemical test system consisting of nine tests, entitled Enterotube, was evaluated in parallel with conventional tests to determine its value in the identification of enteric and certain other gram-negative bacilli. The 242 bacterial strains studied were from a variety of pathological specimens and from our culture collection. When the results with individual tests represented in both test systems were compared, no discrepancies were noted in the indole test, and one discrepancy was recorded for dextrose. In 7 of 242 hydrogen sulfide tests, 3 of 242 phenylalanine tests, 22 of 242 urease tests, 15 of 242 dulcitol tests, 12 of 242 lactose tests, 27 of 217 lysine decarboxylase tests, and 5 of 242 citrate tests, the Enterotube results were contrary to those obtained with conventional methods. The lysine decarboxylase test in the Enterotube posed a problem of interpretation and readability and is not an acceptable alternative to the conventional methods. Fifteen of the strains studied were incorrectly identified by the Enterotube system and four could not be differentiated from other closely related strains. Salmonella could be identified as to group, whereas Shigella strains were frequently misidentified as Escherichia. The Enterotube method is simple and convenient, and all media are inoculated at once from a single colony. |
| Databáze: | OpenAIRE |
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