2,3,7,8-Tetrachlorodibenzo-p-dioxin induces suppressor of cytokine signaling 2 in murine B cells
| Autor: | Robert B. Crawford, Allison S. Harney, Timothy R. Zacharewski, Darrell R. Boverhof, Elaine Tam, Norbert E. Kaminski |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Lymphoma
B-Cell Polychlorinated Dibenzodioxins medicine.medical_treatment Suppressor of Cytokine Signaling Proteins Biology Cycloheximide chemistry.chemical_compound Mice Cell Line Tumor medicine Cytochrome P-450 CYP1A1 Animals heterocyclic compounds SOCS2 B cell DNA Primers Oligonucleotide Array Sequence Analysis Pharmacology Reporter gene B-Lymphocytes Base Sequence Aryl hydrocarbon receptor Molecular biology DNA-Binding Proteins Repressor Proteins Cytokine medicine.anatomical_structure chemistry Gene Expression Regulation Cancer cell biology.protein Trans-Activators Molecular Medicine Signal transduction Signal Transduction |
| Zdroj: | Molecular pharmacology. 66(6) |
| ISSN: | 0026-895X |
| Popis: | The B cell, a major component of humoral immunity, is a sensitive target for the immunotoxic effects of 2,3,7,8-tetrachlorodibenzo- p -dioxin (TCDD), possibly by rendering cells less responsive to antigenic or mitogenic stimulation. Potential mechanisms of TCDD action on B cells were examined in murine B cell lymphoma cells (CH12.LX) treated with 3 nM TCDD or dimethyl sulfoxide vehicle using sequence-verified cDNA microarrays. One transcript that was significantly induced by TCDD was suppressor of cytokine signaling 2 (Socs2). Changes in Socs2 mRNA levels paralleled that of Cyp1a1 with a maximal 3-fold induction observed at 4 h, as determined by quantitative real-time polymerase chain reaction. Socs2 induction seems B cell-specific, because no induction was observed in TCDD-responsive mouse hepatoma cells or human breast cancer cells. TCDD-mediated induction of Socs2 mRNA was dose-dependent and exhibited the characteristic structure-activity relationships observed for the aryl hydrocarbon receptor (AhR) ligands 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126), indolo[3,2- b ]-carbazole, and β-naphthoflavone. Experiments with cycloheximide and AhR-deficient B cells indicated that Socs2 mRNA induction is a primary effect that is AhR-dependent. Western blot analysis confirmed that Socs2 and Cyp1a1 protein levels were also induced in CH12.LX cells. Promoter analysis revealed the presence of four dioxin-response elements within 1000 base pairs upstream of the Socs2 transcriptional start site, and a reporter gene regulated by the Socs2 promoter was inducible by TCDD. Promoter activity was also dependent on a functional AhR signaling pathway. These results indicate that Socs2 is a primary TCDD-inducible gene that may represent a novel mechanism by which TCDD elicits its immunosuppressive effects. |
| Databáze: | OpenAIRE |
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