The effects of MSH2 deficiency on spontaneous and radiation-induced mutation rates in the mouse germline

Autor: Karen L.-A. Burr, Annemarie van Duyn-Goedhart, Peter T. Hickenbotham, Yuri E. Dubrova, Paul P.W. van Buul, Karen Monger
Rok vydání: 2007
Předmět:
Zdroj: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 617:147-151
ISSN: 0027-5107
DOI: 10.1016/j.mrfmmm.2007.01.010
Popis: Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of mismatch repair deficient Msh2 knock-out mice. Spontaneous mutation rates in homozygous Msh2 −/− males were significantly higher than those in isogenic wild-type ( Msh2 +/+ ) and heterozygous ( Msh2 +/− ) mice. In contrast, the irradiated Msh2 −/− mice did not show any detectable increases in their mutation rate, whereas significant ESTR mutation induction was observed in the irradiated Msh2 +/+ and Msh2 +/− animals. Considering these data and the results of other publications, we propose that the Msh2 -deficient mice possess a mutator phenotype in their germline and somatic tissues while the loss of a single Msh2 allele does not affect the stability of heterozygotes.
Databáze: OpenAIRE