The effects of MSH2 deficiency on spontaneous and radiation-induced mutation rates in the mouse germline
Autor: | Karen L.-A. Burr, Annemarie van Duyn-Goedhart, Peter T. Hickenbotham, Yuri E. Dubrova, Paul P.W. van Buul, Karen Monger |
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Rok vydání: | 2007 |
Předmět: |
Male
Heterozygote congenital hereditary and neonatal diseases and abnormalities Mutation rate Somatic cell Health Toxicology and Mutagenesis Biology Radiation Tolerance Germline Mice Germline mutation Radiation Ionizing Genetics Animals Allele neoplasms Molecular Biology Germ-Line Mutation Mice Knockout Mice Inbred BALB C Homozygote nutritional and metabolic diseases Heterozygote advantage Molecular biology digestive system diseases Mice Inbred C57BL MutS Homolog 2 Protein Tandem Repeat Sequences MSH2 Female DNA mismatch repair |
Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 617:147-151 |
ISSN: | 0027-5107 |
DOI: | 10.1016/j.mrfmmm.2007.01.010 |
Popis: | Mutation rates at two expanded simple tandem repeat (ESTR) loci were studied in the germline of mismatch repair deficient Msh2 knock-out mice. Spontaneous mutation rates in homozygous Msh2 −/− males were significantly higher than those in isogenic wild-type ( Msh2 +/+ ) and heterozygous ( Msh2 +/− ) mice. In contrast, the irradiated Msh2 −/− mice did not show any detectable increases in their mutation rate, whereas significant ESTR mutation induction was observed in the irradiated Msh2 +/+ and Msh2 +/− animals. Considering these data and the results of other publications, we propose that the Msh2 -deficient mice possess a mutator phenotype in their germline and somatic tissues while the loss of a single Msh2 allele does not affect the stability of heterozygotes. |
Databáze: | OpenAIRE |
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