Directed differentiation of human embryonic stem cells to corneal endothelial cell-like cells: A transcriptomic analysis
Autor: | Guoping Fan, Frank Fuxiang Mao, Qinglu Song, Qin An, Yinyin Chen, Songtao Yuan, Yizhi Liu, Qinghuai Liu |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Cell Human Embryonic Stem Cells Biology Corneal Diseases Transcriptome 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Directed differentiation medicine Animals Humans Induced pluripotent stem cell Cells Cultured Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Endothelium Corneal Neural crest Cell Differentiation Flow Cytometry Embryonic stem cell Sensory Systems In vitro Cell biology Transplantation Ophthalmology 030104 developmental biology medicine.anatomical_structure Immunology cardiovascular system 030221 ophthalmology & optometry RNA Cattle |
Zdroj: | Experimental eye research. 151 |
ISSN: | 1096-0007 |
Popis: | Corneal endothelial cells (CECs) are a monolayer of cells covering the inner-side of cornea, playing a pivotal role in keeping the cornea transparent. Because adult CECs have no proliferative capacity, the loss of CECs during aging or under pathological conditions would lead to corneal edema, eventually leading to the blindness. Clinically, donated CECs have been successfully transplanted to treat the diseases of CEC deficiency; however, the source of CEC donation is very limited. As an alternative cell source for CEC transplantation, CEC-like cells can be obtained via in vitro differentiation of human pluripotent stem cells. In this study, we introduced a modified two-stage differentiation method to convert H9 human embryonic stem cells (hESCs) to neural crest cells (NCCs), then further into CEC-like cells. The CEC-like cells treated with bovine CEC conditional medium morphologically best resembled primary CECs among all the culture conditions. By whole transcriptome analysis, we found that the typical markers of CECs, such as Na+-K+-ATPase, AQP1, Col8a and ZO-1, are highly expressed in hESC-derived CEC-like cells. By comparing RNA transcriptome of hESC-derived CEC-like cells with human primary fetal and adult CECs, we further identified shared molecular markers such as TRIT1, HSPB11, CRY1 that can be used to quality control CEC derivatives from hESCs. Our study paves the way for the quality-control and future application of hESC-derived CECs in the treatment of CEC deficiency disorders. |
Databáze: | OpenAIRE |
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