Angiogenic functionalisation of titanium surfaces using nano-anchored VEGF – an in vitro study
| Autor: | Henning Schliephake, N Strecker, Bernd Schwenzer, Anne Förster, Judith Reichert, Dieter Scharnweber |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Vascular Endothelial Growth Factor A
lcsh:Diseases of the musculoskeletal system Surface Properties medicine.medical_treatment Oligonucleotides lcsh:Surgery Neovascularization Physiologic 02 engineering and technology Conjugated system In Vitro Techniques recombinant proteins Umbilical vein 03 medical and health sciences chemistry.chemical_compound angiogenesis 0302 clinical medicine Von Willebrand factor von Willebrand Factor parasitic diseases medicine Human Umbilical Vein Endothelial Cells Humans titanium Cells Cultured Cell Proliferation biology Oligonucleotide Growth factor Mesenchymal stem cell Vascular endothelial growth factor nucleotides controlled release Biological activity Mesenchymal Stem Cells 030206 dentistry lcsh:RD1-811 respiratory system 021001 nanoscience & nanotechnology Molecular biology Immobilized Proteins chemistry Delayed-Action Preparations biology.protein lcsh:RC925-935 0210 nano-technology |
| Zdroj: | European Cells & Materials, Vol 23, Pp 161-169 (2012) |
| ISSN: | 1473-2262 |
| Popis: | The aim of the present study was to test the hypothesis that sandblasted and acid etched titanium surfaces can be functionalised with vascular endothelial growth factor (VEGF) using oligonucleotides for anchorage and slow release. rhVEGF165 molecules were conjugated to strands of 30-mer non-coding DNA oligonucleotides (ODN) and hybridised to complementary ODN anchor strands which had been immobilised to the surface of sandblasted/acid etched (SAE) Ti specimens. Specimens with non-conjugated VEGF adsorbed to ODN anchor strands and to blank SAE surfaces served as controls. Specifi c binding of conjugated VEGF exhibited the highest percentage of immobilised VEGF (71.0 %), whereas non-conjugated VEGF only achieved 53.2 and 30.7 %, respectively. Cumulative release reached 54.0 % of the immobilised growth factor in the group of specifi cally bound VEGF after 4 weeks, whereas non-conjugated VEGF adsorbed to ODN strands released 78.9 % and VEGF adsorbed to SAE Ti surfaces released 97.4 %. Proliferation of human umbilical vein endothelial cells (HUVECs) was signifi cantly increased on the surfaces with specifi cally bound VEGF compared to the control surfaces and SAE Ti surfaces without VEGF. Moreover, the released conjugated VEGF exhibited biological activity by induction of von Willebrand Factor (vWF) in mesenchymal stem cells. It is concluded that the angiogenic functionalisation of SAE titanium surfaces can be achieved by conjugation of VEGF to ODN strands and hybridisation to complementary ODN strands that are anchored to the titanium surface. The angiogenic effect is exerted both through the immobilised and the released portion of the growth factor. peerReviewed |
| Databáze: | OpenAIRE |
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