A high-throughput media design approach for high performance mammalian fed-batch cultures
| Autor: | Matthieu Stettler, Natacha Collet, Hervé Broly, Martin Jordan, Arnaud Périlleux, Yolande Rouiller |
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| Rok vydání: | 2013 |
| Předmět: |
Design–Expert
Computer science Immunology CHO Cells Bioreactors Cricetulus Software Report Animals Humans Immunology and Allergy Media design Process engineering Throughput (business) Mammals business.industry Design of experiments Process (computing) Culture Media High-Throughput Screening Assays Biotechnology Identification (information) Glucose Ranking Batch Cell Culture Techniques business |
| Zdroj: | mAbs. 5:501-511 |
| ISSN: | 1942-0870 1942-0862 |
| DOI: | 10.4161/mabs.23942 |
| Popis: | An innovative high-throughput medium development method based on media blending was successfully used to improve the performance of a Chinese hamster ovary fed-batch medium in shaking 96-deepwell plates. Starting from a proprietary chemically-defined medium, 16 formulations testing 43 of 47 components at 3 different levels were designed. Media blending was performed following a custom-made mixture design of experiments considering binary blends, resulting in 376 different blends that were tested during both cell expansion and fed-batch production phases in one single experiment. Three approaches were chosen to provide the best output of the large amount of data obtained. A simple ranking of conditions was first used as a quick approach to select new formulations with promising features. Then, prediction of the best mixes was done to maximize both growth and titer using the Design Expert software. Finally, a multivariate analysis enabled identification of individual potential critical components for further optimization. Applying this high-throughput method on a fed-batch, rather than on a simple batch, process opens new perspectives for medium and feed development that enables identification of an optimized process in a short time frame. |
| Databáze: | OpenAIRE |
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