Prenatal diagnosis of β thalassaemia by reverse phase HPLC
| Autor: | Yves Beuzard, R. S. Mibashan, Michel Vidaud, Philippe Rouyer-Fessard, P. John |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Heterozygote
medicine.medical_specialty Prenatal diagnosis β thalassaemia High-performance liquid chromatography Pregnancy Prenatal Diagnosis medicine Humans Globin Chromatography High Pressure Liquid Genetics (clinical) Chromatography Fetus business.industry Homozygote Obstetrics and Gynecology Reversed-phase chromatography Surgery CMC chromatography Fetal Diseases Beta-thalassaemia Carboxymethylcellulose Sodium Thalassemia Female business |
| Zdroj: | Prenatal Diagnosis. 7:171-178 |
| ISSN: | 1097-0223 0197-3851 |
| DOI: | 10.1002/pd.1970070304 |
| Popis: | Reverse phase HPLC of radioactive globin chains has been compared to classical carboxy methyl cellulose chromatography for the prenatal diagnosis of beta thalassaemia. The two methods correlated highly (r = 0.97 p less than 0.0005) and provided an identical diagnosis for 40 fetal blood samples of fetuses homozygous or heterozygous for beta thalassaemia. The HPLC procedure was much faster and required fewer biochemical steps (no globin preparation). It was at least as accurate and more sensitive than the classical chromatography. A single column can be used for 150 analyses and is always ready to be used. Last but not least it is much less expensive than CMC chromatography. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text