Identification and Sequence Determination of mRNAs Detected in Dormant (Diapausing) Aedes triseriatus Mosquito Embryos
| Autor: | Bradley J. Blitvich, Carol D. Blair, A. Rayms-Keller, Barry J. Beaty |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
DNA
Complementary Embryo Nonmammalian Base Sequence biology Sequence Analysis RNA Sequence analysis Protein subunit Molecular Sequence Data Ribosomal RNA biology.organism_classification Biochemistry Molecular biology Endocrinology Aedes Complementary DNA Genetics Melanogaster Animals Amino Acid Sequence RNA Messenger Drosophila melanogaster Molecular Biology Peptide sequence Caenorhabditis elegans |
| Zdroj: | DNA Sequence. 12:197-202 |
| ISSN: | 1042-5179 |
| DOI: | 10.3109/10425170109080775 |
| Popis: | Many insects survive adverse climatic conditions in a dormant state known as diapause. In this study, we identified and sequenced several mRNAs in diapausing Aedes triseriatus mosquito embryos. Using reverse-transcription PCR and 5' RACE, we identified a 995-nucleotide cDNA that encodes a 259-amino acid protein of unknown function. This putative protein displays strong sequence similarity to Drosophila melanogaster (95%), human (87%), Caenorhabditis elegans (86%) and yeast (81%) counterparts. The second identified full-length cDNA consists of 624 nucleotides and encodes a 174-amino acid protein of unknown function. This putative protein displays significant sequence similarity to D. melanogaster (68%), human (59%), plant (57%) and yeast (49%) counterparts. We also detected a number of cDNA fragments that exhibited significant sequence similarity to a mitochondrial cytochrome C oxidase subunit, human N33 protein (a potential human prostate tumor suppressor), 18S and 28S ribosomal RNAs, protein disulfide-isomerase, and guanine nucleotide-binding protein. |
| Databáze: | OpenAIRE |
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