Genetically engineered thermotolerant facultative anaerobes for high-efficient degradation of multiple hazardous nitroalkanes
| Autor: | Linbo Sun, Chenchen Peng, Mingchang Li, Di Huang, Wei Wang, Song Xiaoru, Bingling Zhang |
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| Rok vydání: | 2021 |
| Předmět: |
Environmental Engineering
Health Toxicology and Mutagenesis 0211 other engineering and technologies 02 engineering and technology 010501 environmental sciences 01 natural sciences Bioreactors Bioremediation Alkanes Nitroalkane oxidase Bioreactor Environmental Chemistry Food science Waste Management and Disposal 0105 earth and related environmental sciences Pollutant 021110 strategic defence & security studies Facultative Bacteria Chemistry Thermophile Biodegradation Pollution Biodegradation Environmental Genetic Engineering Anaerobic exercise |
| Zdroj: | Journal of Hazardous Materials. 405:124253 |
| ISSN: | 0304-3894 |
| Popis: | Nitroalkanes are important industrial raw materials but also toxic pollutants, which are difficult to degrade once released into the environment. In this study, to significantly improve the degradation-efficiency of multiple nitroalkanes, a facultative anaerobe was genetically engineered, possible influencing factors and simulated application experiments of bioreactor were tested and evaluated. Among all engineered recombinants, the most effective strains NG-S1 (anaerobic) and NG-S2 (aerobic) displayed 2-fold and 2.8-fold final degradation rates higher than the wild type, respectively. Exogenous components, particularly those that enhance coenzyme synthesis, helped to increase the degradation rate, as the level of coenzymes affected full function of overexpressed nitroalkane oxidase. Importantly, simulated mixed-nitroalkane-wastewater bioreactor experiments proved excellent and sustainable degradation performance of the engineered strains for potential industrial applications. Collectively, these findings provide a promising thermophilic biological engineering platform and a new perspective for high-efficient and continuous environmental bioremediation of hazardous pollutants under aerobic and anaerobic conditions. |
| Databáze: | OpenAIRE |
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