Characterization of Neurospora crassa mutant stratins deficient in adenylate cyclase activity
| Autor: | Mirtha M. Flawiá, Héctor N. Torres, Héctor F. Terenzi |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
chemistry.chemical_classification
Mutation Membranes Neurospora crassa Mutant Biophysics Adenylate kinase Biology biology.organism_classification medicine.disease_cause Biochemistry Cyclase Molecular biology Neurospora Enzyme chemistry medicine Specific activity Molecular Biology Cyclase activity Crosses Genetic Adenylyl Cyclases |
| Zdroj: | Archives of biochemistry and biophysics. 180(2) |
| ISSN: | 0003-9861 |
| Popis: | Mycelial extracts from Neurospora crassa strains having any one of five different mutations of the cr-1 allele (“crisp”) exhibited an adenylate cyclase specific activity 2 to 3% of that found in wild-type strains. The enzyme deficiency seemed to be specific for the cr-1 mutation but not for the “crisp” morphology and recessive in heterocaryons carrying mutated and wild-type cr-1 alleles. The reduced adenylate cyclase activity detected in extracts from cr-1 mutants was not due to an impairment in the extraction of membranes, to a preferential inactivation of the enzyme after extraction, or to the presence of inhibitors. |
| Databáze: | OpenAIRE |
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