Gut-specific arylphorin mediates midgut regenerative response against Cry-induced damage in Achaea janata
| Autor: | Aparna Dutta-Gupta, Vivek Thakur, R.K. Chaitanya, Dasari Abhilash, Narender K. Dhania, Shourya Dutta-Gupta, Vinod K. Chauhan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Physiology Cyclin B Bacillus thuringiensis Biology Moths Biochemistry 03 medical and health sciences 0302 clinical medicine RNA interference Animals Molecular Biology Mitosis Gene knockdown Bacillus thuringiensis Toxins Regeneration (biology) fungi Midgut biology.organism_classification Cell biology Intestines 030104 developmental biology biology.protein Insect Proteins Stem cell 030217 neurology & neurosurgery Achaea janata |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 255 |
| ISSN: | 1879-1107 |
| Popis: | Development of insect resistance to biopesticides is a current and pertinent global issue. Earlier, it was established that lepidopteran larvae can recover from Bt intoxication via a midgut regenerative response and subsequently generate resistance. Molecular aspects of restoration of the midgut integrity following toxin exposure are emerging recently. In the present study, we bring out the pivotal role of gut arylphorin in mediating the midgut regenerative response following sublethal Bt exposure in Achaea janata. Bt-induced midgut damage was characterized by microscopic analysis using differential interference contrast (DIC) and immunofluorescence (IF). Extensive disruption of brush-border membrane, associated with the underlying cytoskeletal alterations including F-actin, α-actin and β-tubulin was observed. Single-photon fluorescence microscopy combined with fluorescence lifetime imaging (FLIM) established the metabolic state associated with enhanced stem cell proliferation and migration from the basal side towards the luminal side following the damage. In-silico analysis revealed the phylogenetic relationship of gut arylphorin with closely related insect species and indicated the presence of two different subunits. Transient RNAi knockdown of the arylphorin resulted in diminished expression of mitotic Cyclin B mRNA levels. Human monoclonal Cyclin B antibody cross-reactivity with the Cyclin B located in the stem cells further validate the role of arylphorin as the mitogenic factor responsible for stem cell proliferation and epithelial regeneration. An in-depth understanding of resistance mechanisms will aid in the design of new strategies for the long-term usage and efficacy of Bt technology against pest control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect