Adenosine A2B receptor activation stimulates glucose uptake in the mouse forebrain
Autor: | Bárbara S. Pinheiro, Rui O. Beleza, Cristina Lemos, Ricardo Gouveia Rodrigues, Attila Köfalvi, Joana Marques, Rodrigo A. Cunha, Daniel Rial |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Male
medicine.medical_specialty Adenosine A2 Receptor Agonists Glucose uptake Hippocampal formation Biology Deoxyglucose In Vitro Techniques Receptor Adenosine A2B Hippocampus Cellular and Molecular Neuroscience chemistry.chemical_compound Mice Adenosine deaminase Prosencephalon Internal medicine medicine Animals Lactic Acid Molecular Biology Cells Cultured Mice Knockout Neurons Glycogen Glucose transporter Cell Biology Adenosine receptor Adenosine Mice Inbred C57BL Endocrinology 4-Chloro-7-nitrobenzofurazan Glucose chemistry Astrocytes biology.protein Original Article Adenosine A2B receptor medicine.drug |
Popis: | ATP consumption during intense neuronal activity leads to peaks of both extracellular adenosine levels and increased glucose uptake in the brain. Here, we investigated the hypothesis that the activation of the low-affinity adenosine receptor, the A2B receptor (A(2B)R), promotes glucose uptake in neurons and astrocytes, thereby linking brain activity with energy metabolism. To this end, we mapped the spatiotemporal accumulation of the fluorescent-labelled deoxyglucose, 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG), in superfused acute hippocampal slices of C57Bl/6j mice. Bath application of the A(2B)R agonist BAY606583 (300 nM) triggered an immediate and stable (>10 min) increase of the velocity of 2-NBDG accumulation throughout hippocampal slices. This was abolished with the pretreatment with the selective A(2B)R antagonist, MRS1754 (200 nM), and was also absent in A(2B)R null-mutant mice. In mouse primary astrocytic or neuronal cultures, BAY606583 similarly increased (3)H-deoxyglucose uptake in the following 20 min incubation period, which was again abolished by a pretreatment with MRS1754. Finally, incubation of hippocampal, frontocortical, or striatal slices of C57Bl/6j mice at 37 °C, with either MRS1754 (200 nM) or adenosine deaminase (3 U/mL) significantly reduced glucose uptake. Furthermore, A(2B)R blockade diminished newly synthesized glycogen content and at least in the striatum, increased lactate release. In conclusion, we report here that A(2B)R activation is associated with an instant and tonic increase of glucose transport into neurons and astrocytes in the mouse brain. These prompt further investigations to evaluate the clinical potential of this novel glucoregulator mechanism. |
Databáze: | OpenAIRE |
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