Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya
| Autor: | Yasuhiko Kawakami, Juan Carlos Izpisua Belmonte, Isao Oishi, Angel Raya, Carles Callol-Massot |
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| Rok vydání: | 2006 |
| Předmět: |
Embryo
Nonmammalian Organogenesis Morphogenesis Animals Genetically Modified Microtubule Ciliogenesis Genetics Animals Cilia Axis specification Cloning Molecular Zebrafish Tissue homeostasis Body Patterning biology Cilium Intracellular Signaling Peptides and Proteins Wnt signaling pathway Gene Expression Regulation Developmental Zebrafish Proteins biology.organism_classification Cell biology Wnt Proteins Signal Transduction |
| Zdroj: | Nature Genetics. 38:1316-1322 |
| ISSN: | 1546-1718 1061-4036 |
| DOI: | 10.1038/ng1892 |
| Popis: | Primary cilia are microtubule-based organelles that project from the surface of nearly every animal cell. Although important functions of primary cilia in morphogenesis and tissue homeostasis have been identified, the mechanisms that control the formation of primary cilia are not understood. Here we characterize a zebrafish gene, termed duboraya (dub), that is essential for ciliogenesis. Knockdown of dub in zebrafish embryos results in both defects in primary cilia formation in Kupffer's vesicle and randomization of left-right organ asymmetries. We show that, at the molecular level, the function of dub in ciliogenesis is regulated by phosphorylation, which in turn depends on Frizzled-2-mediated noncanonical Wnt signaling. We also provide evidence that, at the cellular level, dub function is essential for actin organization in the cells lining Kupffer's vesicle. Taken together, our findings identify a molecular factor that links noncanonical Wnt signaling with the control of left-right axis specification, and provide an entry point for analyzing the mechanisms that regulate primary cilia formation. |
| Databáze: | OpenAIRE |
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