A role for the Saccharomyces cerevisiae ABCF protein New1 during translation termination
| Autor: | Victoriia Murina, Agnieszka A. Pochopien, Michael Graf, Yang Zhou, Tonu Margus, Jiří Nováček, Marcus J.O. Johansson, Villu Kasari, Tracy Nissan, Daniel N. Wilson, Vasili Hauryliuk, Gemma C. Atkinson |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Chemistry Translation (biology) Ribosome Ribosome assembly Cell biology Elongation factor 03 medical and health sciences 0302 clinical medicine Protein biosynthesis Ribosome profiling Translation factor Eukaryotic Ribosome 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/638064 |
| Popis: | Translation on the ribosome is controlled by numerous accessory proteins and translation factors. In the yeastSaccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related ABCF ATPase, New1, is encoded by a non-essential gene with a cold sensitivity and ribosome assembly defect knock-out phenotype. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is abona fideribosome assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed a combination of yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3’-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination. |
| Databáze: | OpenAIRE |
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