Electroacupuncture ameliorates abnormal defaecation and regulates corticotrophin-releasing factor in a rat model of stress
Autor: | Xiao-Chun Yu, Yu-Xue Zhao, Changxiang Cui, Bing Zhu, Juan-Juan Xin, Feng-Yan Lu, Jun-Hong Gao |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty Electroacupuncture Corticotropin-Releasing Hormone medicine.medical_treatment Abnormal defaecation Rat model Hypothalamus Bowen's Disease Hindlimb Rats Sprague-Dawley 03 medical and health sciences Corticotropin-releasing hormone 0302 clinical medicine Stress Physiological Internal medicine hemic and lymphatic diseases medicine Acupuncture Animals Humans skin and connective tissue diseases Defecation Original Paper integumentary system business.industry General Medicine Rats Blot Disease Models Animal 030104 developmental biology Endocrinology Complementary and alternative medicine 030211 gastroenterology & hepatology Neurology (clinical) business Acupuncture Points |
Zdroj: | Acupuncture in Medicine |
ISSN: | 1759-9873 0964-5284 |
Popis: | Objective To examine the effect of electroacupuncture (EA) treatment on abnormal defaecation in a rat model of chronic heterotypic stress (CHS) and investigate the underlying mechanisms. Methods 20 male Sprague-Dawley rats were randomly divided into three groups: normal (n=6), CHS (n=7), and CHS+EA (n=7). Rats in the CHS group and CHS+EA groups received four different types of stressors for 7 days. For rats in the CHS+EA group, EA was applied at ST36 in the bilateral hind legs for 30 min before each stress-loading session. Rats in the normal group did not receive stressors or EA treatment. The faecal pellets of each rat were collected and weighed at a fixed time every day. Protein expression of corticotrophin-releasing factor (CRF) in the hypothalamus and colorectal tissues was measured by Western blotting at the end of the experiment on the 7th day. Results After 7 consecutive days of CHS, the number of faecal pellets, faecal wet weight, and faecal water content were significantly increased in the CHS group compared with the normal group (p=0.035, p=0.008 and p=0.008, respectively). All three parameters were significantly decreased in CHS+EA versus CHS groups (p=0.030, p=0.011 and p=0.006, respectively). Stress significantly increased CRF expression in both the hypothalamus and colorectal tissues. The excessive CRF responses seen following CHS were significantly suppressed by EA treatment. Conclusions EA treatment can ameliorate stress loading induced abnormal defaecation in rats and decrease protein expression of CRF centrally (hypothalamus) and peripherally (colorectal tissues), suggesting a potentially therapeutic role for EA in stress-related responses. |
Databáze: | OpenAIRE |
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