Detection of P‐glycoprotein in frozen and paraffin‐embedded gastric adenocarcinoma tissues using a panel of monoclonal antibodies
| Autor: | Maria V. Camarasa, Mayol Mj, Calpena R, Lacueva Fj, Teruel A, Pérez-Vázquez Mt, José A. Ferragut, J Medrano, Rufete C |
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| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty Histology medicine.drug_class Adenocarcinoma Monoclonal antibody Pathology and Forensic Medicine Stomach Neoplasms Tumor Cells Cultured Frozen Sections Humans Medicine Neoplasm ATP Binding Cassette Transporter Subfamily B Member 1 Frozen section procedure Paraffin Embedding biology business.industry Stomach Antibodies Monoclonal General Medicine medicine.disease Immunohistochemistry Staining medicine.anatomical_structure biology.protein Antibody business |
| Zdroj: | Histopathology. 32:328-334 |
| ISSN: | 1365-2559 0309-0167 |
| DOI: | 10.1046/j.1365-2559.1998.00381.x |
| Popis: | Aims: Most chemotherapeutic regimens used against gastric carcinoma include anthracyclines whose effectiveness can be impaired by the presence of P-glycoprotein. In order to obtain a reliable pattern of P-glycoprotein expression in these tumours an immunohistochemical study using a panel of anti-P-glycoprotein antibodies was performed in frozen and paraffinized tissues. Methods and results: Frozen and paraffinized samples from 25 gastric carcinomas were immunohistochemically analysed using a panel of four anti-P-glycoprotein monoclonal antibodies including C219, MRK16, JSB-1 and C494. Semiquantitative analysis indicated that moderate or high P-glycoprotein levels were detected in 40% to 76% of gastric adenocarcinomas, depending on the anti-P-glycoprotein antibody used. The antibody C494 was the most sensitive in detecting P-glycoprotein in both frozen and paraffinized gastric carcinoma samples. Moreover, C494 showed a pattern of staining exclusively associated with the plasma membrane, in contrast to the cytoplasmic with reinforcement of plasma membrane pattern displayed by the other three antibodies. Significant differences in P-glycoprotein levels were obtained when C494 and MRK16 were used in frozen tissues. Finally, detection of P-glycoprotein in frozen samples did not improve when compared to paraffinized ones. Conclusions: It appears that P-glycoprotein is frequently expressed in gastric adenocarcinomas, and the use of C494 complemented by JSB-1 is recommended for reliable detection of P-glycoprotein in this neoplasm. |
| Databáze: | OpenAIRE |
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