Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice
| Autor: | John F. Disterhoft, Craig Weiss, Carmen Lin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
genetic structures Computer science Head (linguistics) General Chemical Engineering Hippocampus Stimulation Cellular level General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Animals Behavior General Immunology and Microbiology General Neuroscience Classical conditioning Electrical shock Conditioning Eyelid Electric Stimulation 030104 developmental biology Eyeblink conditioning Vibrissae Models Animal Conditioning Head Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Eyeblink conditioning is a common paradigm for investigating the neural mechanisms underlying learning and memory. To better utilize the extensive repertoire of scientific techniques available to study learning and memory at the cellular level, it is ideal to have a stable cranial platform. Because mice do not readily tolerate restraint, they are usually trained while moving about freely in a chamber. Conditioned stimulus (CS) and unconditioned stimulus (US) information are delivered and eyeblink responses recorded via a tether connected to the mouse's head. In the head-fixed apparatus presented here, mice are allowed to run as they desire while their heads are secured to facilitate experimentation. Reliable conditioning of the eyeblink response is obtained with this training apparatus, which allows for the delivery of whisker stimulation as the CS, a periorbital electrical shock as the US, and analysis of electromyographic (EMG) activity from the eyelid to detect blink responses. |
| Databáze: | OpenAIRE |
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