Bandeiraea (Griffonia) simplicifolialectin-I, isolectin A4, reacting with Tn (GalNAαl→ Ser/Thr or galabiose (Galα1 → 4Gal) containing ligands

Ser/Thr) sequence or human blood group Pk active disaccharide (E, Gal alpha1 --> 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS I-A4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A4 interaction was inhibited by both Tn containing glycopeptides and Gal alpha1 --> 4Gal indicating that GS I-A4 not only reacts with human blood group A(GalNAc alpha1 --> 3Gal) and B(Gal alpha1 --> 3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Gal alpha1 --> 4-Gal) ligand. From these results, the carbohydrate specificity of GS I-A4 can be defined as A, Tn > or = B and E. -->

ISSN:	0014-5793
DOI:	10.1016/s0014-5793(96)01227-6
Přístupová URL adresa:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a835c687b7b5bc5941789593cac21b76 https://doi.org/10.1016/s0014-5793(96)01227-6
Rights:	OPEN
Přírůstkové číslo:	edsair.doi.dedup.....a835c687b7b5bc5941789593cac21b76
Autor:	M Wu Albert, Shuh-Chyung Song, H Wu June, Elvin A. Kabat
Rok vydání:	1996
Předmět:	chemistry.chemical_classification Sheep hydatid cyst glycoprotein biology Carbohydrate chemistry Biophysics Disaccharide Lectin Cell Biology Carbohydrate Binding property biology.organism_classification Precipitin Biochemistry Glycopeptide Griffonia chemistry.chemical_compound chemistry Tn-active glycoprotein Structural Biology Genetics biology.protein Bandeiraea (Griffonia) simplicifolia lectin-I A4 Glycoprotein Molecular Biology
Zdroj:	FEBS Letters. 398:183-186
ISSN:	0014-5793
DOI:	10.1016/s0014-5793(96)01227-6
Popis:	Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAc alpha1 --> Ser/Thr) sequence or human blood group Pk active disaccharide (E, Gal alpha1 --> 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS I-A4 reacted strongly with both the Tn active glycoproteins purified from asialo porcine, ovine and armadillo submandibular glands and a P1 active glycoprotein isolated from sheep hydatid fluid. They precipitated over 80% of the lectin nitrogen added. The asialo porcine salivary glycoprotein-GS I-A4 interaction was inhibited by both Tn containing glycopeptides and Gal alpha1 --> 4Gal indicating that GS I-A4 not only reacts with human blood group A(GalNAc alpha1 --> 3Gal) and B(Gal alpha1 --> 3Gal) active disaccharides, but also recognizes the Tn sequence and the E(Gal alpha1 --> 4-Gal) ligand. From these results, the carbohydrate specificity of GS I-A4 can be defined as A, Tn > or = B and E.
Databáze:	OpenAIRE
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