Dopamine receptor D1- and D2-agonists do not spark brown adipose tissue thermogenesis in mice

Autor:	Jens Mittag, Julia Resch, Francesca-Maria Raffaelli, Rebecca Oelkrug, K. Alexander Iwen
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Agonist Male medicine.medical_specialty medicine.drug_class Adipose tissue lcsh:Medicine Biology Article Mice Dopamine receptor D1 Adipose Tissue Brown In vivo Internal medicine Brown adipose tissue medicine Animals Homeostasis Obesity lcsh:Science Multidisciplinary Receptors Dopamine D2 Receptors Dopamine D1 lcsh:R Thermogenesis Endocrinology medicine.anatomical_structure Dopamine receptor Thermography Dopamine Agonists Benzimidazoles lcsh:Q 2 3 4 5-Tetrahydro-7 8-dihydroxy-1-phenyl-1H-3-benzazepine Fat metabolism Ex vivo Signal Transduction
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports
ISSN:	2045-2322
DOI:	10.1038/s41598-020-77143-6
Popis:	Brown adipose tissue (BAT) thermogenesis is considered a potential target for treatment of obesity and diabetes. In vitro data suggest dopamine receptor signaling as a promising approach; however, the biological relevance of dopamine receptors in the direct activation of BAT thermogenesis in vivo remains unclear. We investigated BAT thermogenesis in vivo in mice using peripheral administration of D1-agonist SKF38393 or D2-agonist Sumanirole, infrared thermography, and in-depth molecular analyses of potential target tissues; and ex vivo in BAT explants to identify direct effects on key thermogenic markers. Acute in vivo treatment with the D1- or D2-agonist caused a short spike or brief decrease in BAT temperature, respectively. However, repeated daily administration did not induce lasting effects on BAT thermogenesis. Likewise, neither agonist directly affected Ucp1 or Dio2 mRNA expression in BAT explants. Taken together, the investigated agonists do not seem to exert lasting and physiologically relevant effects on BAT thermogenesis after peripheral administration, demonstrating that D1- and D2-receptors in iBAT are unlikely to constitute targets for obesity treatment via BAT activation.
Databáze:	OpenAIRE
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