Comparison between two kinds of cigarette smoke condensates (CSCs) of the cytogenotoxicity and protein expression in a human B-cell lymphoblastoid cell line using CCK-8 assay, comet assay and protein microarray
| Autor: | Juanjuan Xu, Xiaoxue Li, Guojun Zhou, Jianlin Lou, Jiliang He, Jian Jiang, Shu Zheng, Wei Jiang, Fangfang Huang, Yezhen Lu, Zhijian Chen, Guohai Chu |
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| Rok vydání: | 2009 |
| Předmět: |
Antibody microarray
Cell Survival Health Toxicology and Mutagenesis Protein Array Analysis Biology Sincalide Cell Line Nicotine Tar (tobacco residue) Western blot Genetics medicine Humans B-Lymphocytes medicine.diagnostic_test Mutagenicity Tests Lymphoblast Gene Expression Profiling Molecular biology Comet assay Cell culture Protein microarray Tobacco Smoke Pollution Comet Assay Biomarkers medicine.drug |
| Zdroj: | Mutation research. 697(1-2) |
| ISSN: | 0027-5107 |
| Popis: | The differences of the cytogenotoxicity and proteins expression of human B-cell lymphoblastoid cells exposed to cigarette smoke condensates (CSCs) from two kinds of cigarettes were detected with CCK-8 assay, comet assay, protein microarray and western blot assay in vitro. Human B-cell lymphoblastoid cell line was exposed to CSCs from two cigarettes (which delivers approximately 3 mg tar, 0.3 mg nicotine, 3 mg CO per cigarette for cigarette 1 and 15 mg tar, 1.3 mg nicotine, 15 mg CO per cigarette for cigarette 2), and the exposure doses were 2.5, 5.0, 7.5, 10.0 and 12.5 × 10−3 cigarettes/ml of CSCs for 24 h in CCK-8 assay, 6.0, 8.0, 10.0, 12.0 and 14.0 × 10−3 cigarettes/ml of CSCs for 4 h in comet assay, and 10.0 × 10−3 cigarettes/ml of CSCs for 4 h in protein levels analysis. The results of CCK-8 assay and comet assay in the present study suggested that the cytogenotoxicity in cigarette 2 group was significantly higher than that in cigarette 1 group. The results of protein microarray and western blot assay showed that there were the differences of the expression levels of four proteins (i.e., RAR-β, 14-3-3 sigma, XPF, and p57Kip2 Ab-7) between cigarette 1 group and cigarette 2 group. Hence, it is possible that the RAR-β, 14-3-3 sigma, XPF, and p57Kip2 Ab-7 proteins serve as the molecular biomarkers in studying the cytogenotoxicity induced by CSCs. |
| Databáze: | OpenAIRE |
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