Cytogenetic Elaboration of a Novel Reciprocal Translocation in Sheep
Autor: | D. Di Berardino, Pietro Parma, L. De Lorenzi, Alessandra Iannuzzi, V. Genualdo, Leopoldo Iannuzzi, Angela Perucatti |
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Přispěvatelé: | Iannuzzi, A., Perucatti, A., Genualdo, V., De Lorenzi, L., DI BERARDINO, Dino, Parma, P., Iannuzzi, Leopoldo |
Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Male
sheep Young sheep Karyotype translocation Chromosomal translocation Translocation Genetic Centromere Genetics Animals Molecular Biology Ovis In Situ Hybridization Fluorescence Sheep Domestic Genetics (clinical) biology Reproducibility of Results biology.organism_classification Chromosomes Mammalian Phenotype Chromosome Banding Telomere Karyotyping Cytogenetic Analysis Hybridization Genetic Fish Female DNA Probes |
Zdroj: | Cytogenetic and genome research (Print. ed.) 139 (2013): 97–101. doi:10.1159/000346193 info:cnr-pdr/source/autori:A. Iannuzzi, A. Perucatti, V. Genualdo, L. De Lorenzi, D. Di Berardino, P. Parma, L. Iannuzzi/titolo:Cytogenetic Elaboration of a Novel Reciprocal Translocation in Sheep/doi:10.1159%2F000346193/rivista:Cytogenetic and genome research (Print. ed.)/anno:2013/pagina_da:97/pagina_a:101/intervallo_pagine:97–101/volume:139 |
DOI: | 10.1159/000346193 |
Popis: | Reciprocal translocations represent one of the most common structural chromosomal rearrangements observed in both humans and domestic animals. In these translocations, the balanced forms are most frequent but may remain undetected because the carriers show a normal phenotype. For this reason, routine cytogenetic analysis of domestic animals should necessarily rely on banded karyotypes. In fact, during a screening analysis, carried out on phenotypically normal young sheep (Ovis aries, OAR, 2n = 54) from Laticauda-Comisana hybrids, a new structural rearrangement was detected. Two abnormal acrocentric chromosomes (the smallest and the largest one) were found in all metaphases of this carrier animal, suggesting the presence of a reciprocal translocation (rcp). CBA and RBA banding were performed in order to characterize the translocation, and FISH with chromosome-specific BAC probes and telomere probes was applied to confirm the cytogenetic data. The translocation was classified as rcp(4q;12q)(q13;q25). |
Databáze: | OpenAIRE |
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