Sevoflurane-induced preconditioning of rat brain in vitro and the role of KATP channels
Autor: | Ralphiel S. Payne, Franz Kehl, Avital Schurr, Norbert Roewer |
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Rok vydání: | 2004 |
Předmět: |
Male
Methyl Ethers Minimum alveolar concentration Potassium Channels Population Ischemia In Vitro Techniques Pharmacology Hippocampal formation Sevoflurane Rats Sprague-Dawley Adenosine Triphosphate Potassium Channel Blockers medicine Animals Hippocampus (mythology) Ischemic Preconditioning education Molecular Biology education.field_of_study Dose-Response Relationship Drug Chemistry General Neuroscience Hypoxia (medical) medicine.disease Rats Anesthesia Anesthetics Inhalation Hypoxia-Ischemia Brain Anesthetic Neurology (clinical) medicine.symptom Hydroxy Acids Decanoic Acids Developmental Biology medicine.drug |
Zdroj: | Brain Research. 1021:76-81 |
ISSN: | 0006-8993 |
Popis: | In the present study we tested the ability of the inhalation anesthetic sevoflurane to induce preconditioning against hypoxia in vitro. Rat hippocampal slices were prepared using established procedures. After 90 min of incubation, slices were exposed for 30 min to 0, 1, 2 or 3 minimum alveolar concentration (MAC) of sevoflurane under normoxic conditions (95% O2/5% CO2). Fifteen minutes later, slices were exposed to 13-min hypoxia (95% N2/5% CO2) followed by 30-min reoxygenation. The amplitude of extracellularly recorded, orthodromically evoked, CA1 population spikes (neuronal function) at the end of the reoxygenation period was measured and used to quantify the degree of recovery of neuronal function posthypoxia. To assess the role that the mitochondrial KATP channel plays in preconditioning, its blocker, 5-hydroxydecanoic acid (5-HD), was added during sevoflurane exposure. Sevoflurane-preconditioning with 1, 2 and 3 MAC increased the degree of recovery of neuronal function after 13-min hypoxia and 30-min reoxygenation from 51 +/- 1% (0 MAC), to 55 +/- 3%, 63 +/- 3%, and 72 +/- 2%, respectively. The effect of 3 MAC sevoflurane was blocked by 5-HD (53 +/- 3%), whereas 5-HD alone had no effect (48 +/- 3%) on the recovery of neuronal function from hypoxia. It is concluded that sevoflurane is capable of inducing preconditioning in vitro in a dose-dependent fashion and involves activation of mitochondrial KATP channels. |
Databáze: | OpenAIRE |
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