Transmission of dynamic supercoiling in linear and multi-way branched DNAs and its regulation revealed by a fluorescent G-quadruplex torsion sensor
| Autor: | Zheng Tan, Yi-de He, Ke-wei Zheng, Yu-hua Hao, Ye Xia, Hong-he Liu, Cui-Jiao Wen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transcription Genetic Regulator Biosensing Techniques Plasma protein binding Biology 010402 general chemistry G-quadruplex 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Structural Biology Genetics A-DNA Promoter Regions Genetic Gene Base Sequence Models Genetic DNA Superhelical DNA Fluorescence 0104 chemical sciences G-Quadruplexes Kinetics Spectrometry Fluorescence 030104 developmental biology chemistry Biophysics DNA supercoil Protein Binding |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gky534 |
| Popis: | DNA supercoiling is an important regulator of gene activity. The transmission of transcription-generated supercoiling wave along a DNA helix provides a way for a gene being transcribed to communicate with and regulate its neighboring genes. Currently, the dynamic behavior of supercoiling transmission remains unclear owing to the lack of a suitable tool for detecting the dynamics of supercoiling transmission. In this work, we established a torsion sensor that quantitatively monitors supercoiling transmission in real time in DNA. Using this sensor, we studied the transmission of transcriptionally generated negative supercoiling in linear and multi-way DNA duplexes. We found that transcription-generated dynamic supercoiling not only transmits along linear DNA duplex but also equally diverges at and proceeds through multi-way DNA junctions. We also show that such a process is regulated by DNA–protein interactions and non-canonical DNA structures in the path of supercoiling transmission. These results imply a transcription-coupled mechanism of dynamic supercoiling-mediated intra- and inter-chromosomal signal transduction pathway and their regulation in DNA. |
| Databáze: | OpenAIRE |
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