Argyrophilic nucleolar organizer regions staining for cytology smears in dogs and cats
| Autor: | Akira Yabuki, Osamu Yamato, Yu Furusawa, Mariko Shima-Sawa, Masashi Takahashi |
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| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Silver Staining air-dried smear counterstain Biology Cat Diseases Dogs Cytology Neoplasms medicine Nucleolus Organizer Region Internal Medicine Animals Agnor staining Dog Diseases CATS General Veterinary fixation argyrophilic nucleolar organizer region Clinical course Counterstain Note Staining Cats cytology Nucleolus organizer region |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | The argyrophilic nucleolar organizer regions (AgNORs) are cellular proliferation markers, crucial for predicting the clinical course and aggressiveness of tumors. The purpose of this study was to establish an easy and practical AgNOR staining method in the cytology of dogs and cats. Air-dried cytological slides were prepared from dogs (n=14) and cats (n=12). Acetone, formalin, ethanol and methanol were tested as fixatives for AgNOR staining. Subsequently, various methods of Romanowsky-based counterstains were tested before and after AgNOR staining. Clear and strong AgNOR spots were observed with all fixatives, and post-May-Grunwald staining was the best counterstaining method. The established method showed clear AgNOR spots even in the long-term storage samples and Romanowsky-stained ones. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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