Absolute Arrangement of Subunits in Cytoskeletal Septin Filaments in Cells Measured by Fluorescence Microscopy

Autor:	Thomas Gronemeyer, Philip Tinnefeld, Charlotte Kaplan, Jürgen J. Schmied, Amy S. Gladfelter, Andrew A. Bridges, Sören Grinhagens, Jonas Ries, Christian M. Winterflood, Patricia Occhipinti, Bo Jing, Helge Ewers
Rok vydání:	2015
Předmět:	Saccharomyces cerevisiae Proteins Bioengineering Saccharomyces cerevisiae macromolecular substances GTPase Septin 03 medical and health sciences 0302 clinical medicine Microscopy Fluorescence microscope DNA origami General Materials Science Cytoskeleton 030304 developmental biology 0303 health sciences Chemistry Mechanical Engineering fungi General Chemistry Condensed Matter Physics Crystallography Single Molecule Microscopy Microscopy Fluorescence Biophysics biological phenomena cell phenomena and immunity Septins 030217 neurology & neurosurgery
Zdroj:	Nano Letters
ISSN:	1530-6992 1530-6984
DOI:	10.1021/acs.nanolett.5b00693
Popis:	We resolved the organization of subunits in cytoskeletal polymers in cells by light microscopy. Septin GTPases form linear complexes of about 32 nm length that polymerize into filaments. We visualized both termini of septin complexes by single molecule microscopy in vitro. Complexes appeared as 32 nm spaced localization pairs, and filaments appeared as stretches of equidistant localizations. Cellular septins were resolved as localization pairs and thin stretches of equidistant localizations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a82b401c6e7cb0337b79a23506a95206 https://doi.org/10.1021/acs.nanolett.5b00693 Zobrazit plný text záznamu