Characterization of weak hydrophobic composite sorbents and their application to the isolation of bacterial lectin
| Autor: | L. S. Zhigis, E.M. Rapoport, Vitaly P. Zubov, O.E. Lisyutina, A.E. Ivanov |
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| Rok vydání: | 1995 |
| Předmět: |
Sialic Acid Binding Immunoglobulin-like Lectins
Sorbent Chromatography biology Composite number Polyacrylamide Acrylic Resins Lectin Proteins General Chemistry Bacillus subtilis biology.organism_classification Silicon Dioxide Surface tension chemistry.chemical_compound chemistry Lectins biology.protein Ammonium Lysozyme Peptides Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical applications. 664(1) |
| ISSN: | 1572-6495 |
| Popis: | Composite sorbents based on wide-pore glass and silica coated with N-butyl polyacrylamide (butyl-PA-glass and butyl-PA-silica) were studied. The surface tension of butyl-PA-silica is 50–55 mJ/m2 as evaluated by the sedimentation volume technique. The linear dependences of log k′ on ammonium sulphate concentration were determined by isocratic chromatography of the dipetide Trp-Trp and lysozyme on butyl-PA-glass. Both solutes were shown to have a weaker retention on butyl-PA-glass than on butyl-Toyopearl 650C. This weaker retention is beneficial in the purification of sialic acid-binding lectin from Bacillus subtilis. |
| Databáze: | OpenAIRE |
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