Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2
| Autor: | Jianhua Sui, Wenhui Li, Wayne A. Marasco, Michael Farzan, Yaqing He, Hyeryun Choe, I-Chueh Huang, Yi Guan, Natalya Vasilieva, Jens H. Kuhn, Shiwen Luo, Chengsheng Zhang, Akikazu Murakami, Keming Xu, Swee-Kee Wong, Michael Moore |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Models
Molecular Recombinant Fusion Proteins Molecular Sequence Data Sequence alignment Carboxypeptidases Plasma protein binding Peptidyl-Dipeptidase A medicine.disease_cause Article General Biochemistry Genetics and Molecular Biology Disease Outbreaks Protein structure Viral Envelope Proteins Viverridae Catalytic Domain medicine Animals Humans Amino Acid Sequence Binding site skin and connective tissue diseases Molecular Biology Peptide sequence Coronavirus Binding Sites Membrane Glycoproteins General Immunology and Microbiology biology General Neuroscience fungi Outbreak Angiotensin-converting enzyme 2 biology.organism_classification Virology Protein Structure Tertiary Rats body regions Severe acute respiratory syndrome-related coronavirus Viral adaptation Severe acute respiratory syndrome Civet Spike Glycoprotein Coronavirus Sequence Alignment hormones hormone substitutes and hormone antagonists Protein Binding |
| Zdroj: | The EMBO Journal |
| Popis: | Human angiotensin-converting enzyme 2 (ACE2) is a functional receptor for SARS coronavirus (SARS-CoV). Here we identify the SARS-CoV spike (S)-protein-binding site on ACE2. We also compare S proteins of SARS-CoV isolated during the 2002-2003 SARS outbreak and during the much less severe 2003-2004 outbreak, and from palm civets, a possible source of SARS-CoV found in humans. All three S proteins bound to and utilized palm-civet ACE2 efficiently, but the latter two S proteins utilized human ACE2 markedly less efficiently than did the S protein obtained during the earlier human outbreak. The lower affinity of these S proteins could be complemented by altering specific residues within the S-protein-binding site of human ACE2 to those of civet ACE2, or by altering S-protein residues 479 and 487 to residues conserved during the 2002-2003 outbreak. Collectively, these data describe molecular interactions important to the adaptation of SARS-CoV to human cells, and provide insight into the severity of the 2002-2003 SARS epidemic. © 2005 European Molecular Biology Organization | All Rights Reserved. published_or_final_version |
| Databáze: | OpenAIRE |
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