Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis

Autor: Chengtao Lu, Juanli Zhang, Jianbo Wang, Li Yan, Hua Li, Yu Zhe, Xiayin Wang, Di Zhang, Aidong Wen
Rok vydání: 2017
Předmět:
Keratinocytes
0301 basic medicine
Aging
Apoptosis
Ataxia Telangiectasia Mutated Proteins
medicine.disease_cause
Biochemistry
Antioxidants
Mice
chemistry.chemical_compound
0302 clinical medicine
Glycosides
Skin
chemistry.chemical_classification
Mice
Inbred BALB C
integumentary system
lcsh:Cytology
General Medicine
8-Hydroxy-2'-Deoxyguanosine
030220 oncology & carcinogenesis
Echinacoside
Oxidoreductases
Research Article
Article Subject
Cell Survival
Ultraviolet Rays
DNA damage
Poly ADP ribose polymerase
DNA Fragmentation
Biology
Cell Line
03 medical and health sciences
medicine
Animals
Humans
lcsh:QH573-671
Reactive oxygen species
L-Lactate Dehydrogenase
Deoxyguanosine
8-Hydroxy-2'-deoxyguanosine
Cell Biology
Molecular biology
Oxidative Stress
HaCaT
030104 developmental biology
chemistry
Reactive Oxygen Species
Oxidative stress
DNA Damage
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
ISSN: 1942-0994
1942-0900
DOI: 10.1155/2017/6851464
Popis: Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage. Cell viability, lactate dehydrogenase (LDH) levels, antioxidant enzyme activities, reactive oxygen species (ROS) generation, DNA damage, and apoptosis were measured as well. Western blot was used to measure the expression of related proteins. The results revealed that pretreatment of echinacoside ameliorated the skin injury; attenuated oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA. To summarize, echinacoside is beneficial in the prevention of UVB-induced DNA damage and apoptosis of the skin in vivo and in vitro.
Databáze: OpenAIRE
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