Influence of HLA-G Polymorphisms In Human Immunodeficiency Virus Infection and hepatitis C Virus Co-Infection in Brazilian and Italian Individuals
| Autor: | Eduardo Sprinz, G.K. da Silva, E. A. A. Cordero, Regina Kuhmmer, Priscila Vianna, Sergio Crovella, Eulalia Catamo, Tiago Degani Veit, Vanessa Suñé Mattevi, Rosmeri Kuhmmer Lazzaretti, José Artur Bogo Chies |
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| Přispěvatelé: | Gabriela Kniphoff da, Silva, Priscila, Vianna, Tiago Degani, Veit, Crovella, Sergio, Catamo, Eulalia, Elvira Alicia Aparicio, Cordero, Vanessa Suñé, Mattevi, Rosmeri Kuhmmer, Lazzaretti, Eduardo, Sprinz, Regina, Kuhmmer, José Artur Bogo, Chies |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Microbiology (medical) Untranslated region Adolescent Hepatitis C virus HLA-G Human immunodeficiency virus (HIV) Black People HIV Infections Human leukocyte antigen Biology medicine.disease_cause Polymorphism Single Nucleotide Microbiology Young Adult Gene Frequency Genotype Genetics medicine Humans Allele 3' Untranslated Regions Molecular Biology Ecology Evolution Behavior and Systematics Aged HLA-G Antigens Coinfection HIV HCV Haplotype Genetic Variation Middle Aged Hepatitis C Virology Infectious Diseases Haplotypes Italy Immunology Brazil |
| Popis: | Objective: This study aimed to investigate the role of Human Leukocyte Antigen (HLA)-G in the susceptibility to HIV-1 infection through the analysis of the HLA-G 30 untranslated region (UTR) polymorphisms 14 bp insertion/deletion (rs66554220) and +3142C>G (rs1063320). Design: We analyzed 582 HIV-1 infected patients and 626 uninfected individuals from Brazil and Italy in a case-control study. Methods: HLA-G polymorphisms were genotyped using PCR, PCR-RFLP assays or direct sequencing. All analyses were stratified by ethnicity. Genotypic, allelic and diplotypic frequencies were compared between HIV-1 infected subjects and controls using Chi-square or Fischer exact tests. Also, haplotypic frequencies were estimated using MLocus software. Results: African-derived HIV-infected individuals presented a higher frequency of the 14 bp insertion allele as compared to non-infected individuals (0.468 versus 0.373, respectively; pBonf = 0.010). A higher frequency of the 14 bp insertion +3142G (insG) haplotype (0.456 versus 0.346, p < 0.001) and the insG/ insG diplotype (OR = 1.88, 95%CI = 1.08–3.23, p = 0.021) was observed among African-derived patients as compared to uninfected controls. Also, we observed a higher frequency of the ins/ins genotype among African-derived HIV patients co-infected with HCV (OR = 2.78, 95%CI = 1.20–6.49, p = 0.008). Conclusions: Our data point out to an increased frequency of alleles and genotypes associated with low HLA-G expression among African-derived patients, suggesting a potential role for HLA-G in the susceptibility to HIV-1 infection and HCV co-infection in those individuals. |
| Databáze: | OpenAIRE |
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