Decreased reuptake of dopamine in the dorsal striatum in the absence of alpha-synuclein
Autor: | Heramb Chadchankar, Jouni Ihalainen, Heikki Tanila, Leonid Yavich |
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Rok vydání: | 2011 |
Předmět: |
Male
Dopamine animal diseases Striatum Synaptic Transmission Reuptake Mice chemistry.chemical_compound 0302 clinical medicine heterocyclic compounds Neurotransmitter Mice Knockout Neurons 0303 health sciences biology General Neuroscience Dopaminergic Parkinson Disease Adaptation Physiological alpha-Synuclein Female medicine.drug medicine.medical_specialty Presynaptic Terminals Down-Regulation Mice Transgenic 03 medical and health sciences Dopamine receptor D1 Dopamine receptor D3 Internal medicine medicine Animals Molecular Biology 030304 developmental biology Dopamine transporter Dopamine Plasma Membrane Transport Proteins organic chemicals Extracellular Fluid nervous system diseases Mice Inbred C57BL Neostriatum Disease Models Animal Endocrinology nervous system chemistry biology.protein Neurology (clinical) 030217 neurology & neurosurgery Developmental Biology |
Zdroj: | Brain Research. 1382:37-44 |
ISSN: | 0006-8993 |
Popis: | The presynaptic protein alpha-synuclein (α-syn) plays a role in dopaminergic neurotransmission in the nigrostriatal dopaminergic system. Mutations in this protein have been linked to pathogenesis of Parkinson's disease. However, the details of regulation of dopamine homeostasis by α-syn and its molecular targets are generally unknown. We investigated the effect of α-syn deletion on striatal dopaminergic homeostasis. Two α-syn deficient mouse lines, one carrying a spontaneous deletion of α-syn locus and the other a transgenic α-syn knockout, were used in the study. Stimulated and basal extracellular dopamine levels were determined in the dorsal striatum by in vivo voltammetry and in vivo microdialysis, respectively. Dopamine transporter expression was studied by immunohistochemistry. Stimulated dopamine overflow and basal extracellular dopamine levels were higher in mice lacking α-syn with a concomitant decrease in dopamine transporter expression and reuptake in the dorsal striatum. We show that α-syn deletion produces significant adaptive changes in the striatal dopaminergic system via modulation of reuptake. |
Databáze: | OpenAIRE |
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