Decreased reuptake of dopamine in the dorsal striatum in the absence of alpha-synuclein

Autor: Heramb Chadchankar, Jouni Ihalainen, Heikki Tanila, Leonid Yavich
Rok vydání: 2011
Předmět:
Male
Dopamine
animal diseases
Striatum
Synaptic Transmission
Reuptake
Mice
chemistry.chemical_compound
0302 clinical medicine
heterocyclic compounds
Neurotransmitter
Mice
Knockout
Neurons
0303 health sciences
biology
General Neuroscience
Dopaminergic
Parkinson Disease
Adaptation
Physiological
alpha-Synuclein
Female
medicine.drug
medicine.medical_specialty
Presynaptic Terminals
Down-Regulation
Mice
Transgenic
03 medical and health sciences
Dopamine receptor D1
Dopamine receptor D3
Internal medicine
medicine
Animals
Molecular Biology
030304 developmental biology
Dopamine transporter
Dopamine Plasma Membrane Transport Proteins
organic chemicals
Extracellular Fluid
nervous system diseases
Mice
Inbred C57BL
Neostriatum
Disease Models
Animal
Endocrinology
nervous system
chemistry
biology.protein
Neurology (clinical)
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Brain Research. 1382:37-44
ISSN: 0006-8993
Popis: The presynaptic protein alpha-synuclein (α-syn) plays a role in dopaminergic neurotransmission in the nigrostriatal dopaminergic system. Mutations in this protein have been linked to pathogenesis of Parkinson's disease. However, the details of regulation of dopamine homeostasis by α-syn and its molecular targets are generally unknown. We investigated the effect of α-syn deletion on striatal dopaminergic homeostasis. Two α-syn deficient mouse lines, one carrying a spontaneous deletion of α-syn locus and the other a transgenic α-syn knockout, were used in the study. Stimulated and basal extracellular dopamine levels were determined in the dorsal striatum by in vivo voltammetry and in vivo microdialysis, respectively. Dopamine transporter expression was studied by immunohistochemistry. Stimulated dopamine overflow and basal extracellular dopamine levels were higher in mice lacking α-syn with a concomitant decrease in dopamine transporter expression and reuptake in the dorsal striatum. We show that α-syn deletion produces significant adaptive changes in the striatal dopaminergic system via modulation of reuptake.
Databáze: OpenAIRE
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