Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion
| Autor: | Randy M. Berka, Daniel S. Rokhsar, Patrik J. Hoegger, Pedro M. Coutinho, José Luis Lavín, Paulo Canessa, Francisco J. Ruiz-Dueñas, Martin Pospíšek, Timothy Y. James, Erika Lindquist, Luis F. Larrondo, Jill Gaskell, Antonio G. Pisabarro, Amber Vanden Wymelenberg, Phil Kersten, Sarah Teter, David S. Hibbett, Grzegorz Sabat, Susan Lucas, Dan Cullen, Patricia Ferreira, Bernard Henrissat, Ángel T. Martínez, Jagjit S. Yadav, José A. Oguiza, Kenneth E. Hammel, Preethi Ramaiya, William Kenealy, Sandra Splinter BonDurant, Harshavardhan Doddapaneni, Christian P. Kubicek, Jean F. Challacombe, Scott E. Baker, Hank Tu, Asaf Salamov, Monika Schmoll, Martin Mokrejs, Kenneth S. Bruno, Ingo Morgenstern, Igor V. Grigoriev, Ursula Kües, Rafael Vicuña, Diego Martinez, Monica Misra, Paul Harris, Emma R. Master, Harris Shapiro, Jon K. Magnuson, Debbie Yaver, Gary Xie, Thomas Brettin, Christine L. Chee, Venkataramanan Subramanian |
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| Přispěvatelé: | Universidad Pública de Navarra. Departamento de Producción Agraria, Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila |
| Rok vydání: | 2009 |
| Předmět: |
Fenton
Glycoside Hydrolases Molecular Sequence Data Cellulase Genome Lignin Transcriptome Postia placenta 03 medical and health sciences chemistry.chemical_compound Cellulases Glycoside hydrolase Cellulose 030304 developmental biology 2. Zero hunger 0303 health sciences Multidisciplinary Base Sequence biology 030306 microbiology Gene Expression Profiling Biological Sciences 15. Life on land biology.organism_classification Biological Evolution Wood Enzymes Wood-decay fungus Brown-Rot chemistry Biochemistry biology.protein Phanerochaete Genome Fungal Oxidoreductases Polyporales Metabolic Networks and Pathways |
| Zdroj: | Digital.CSIC: Repositorio Institucional del CSIC Consejo Superior de Investigaciones Científicas (CSIC) Academica-e. Repositorio Institucional de la Universidad Pública de Navarra instname Academica-e: Repositorio Institucional de la Universidad Pública de Navarra Universidad Pública de Navarra |
| ISSN: | 5504-1825 |
| DOI: | 10.1073/pnas.0809575106 |
| Popis: | Brown-rot fungi such as Postia placenta are common inhabitants of forest ecosystems and are also largely responsible for the destructive decay of wooden structures. Rapid depolymerization of cellulose is a distinguishing feature of brown-rot, but the biochemical mechanisms and underlying genetics are poorly understood. Systematic examination of the P. placenta genome, transcriptome, and secretome revealed unique extracellular enzyme systems, including an unusual repertoire of extracellular glycoside hydrolases. Genes encoding exocellobiohydrolases and cellulose-binding domains, typical of cellulolytic microbes, are absent in this efficient cellulose-degrading fungus. When P. placenta was grown in medium containing cellulose as sole carbon source, transcripts corresponding to many hemicellulases and to a single putative β -1–4 endoglucanase were expressed at high levels relative to glucose-grown cultures. These transcript profiles were confirmed by direct identification of peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Also upregulated during growth on cellulose medium were putative iron reductases, quinone reductase, and structurally divergent oxidases potentially involved in extracellular generation of Fe(II) and H2O2. These observations are consistent with a biodegradative role for Fenton chemistry in which Fe(II) and H2O2 react to form hydroxyl radicals, highly reactive oxidants capable of depolymerizing cellulose. The P. placenta genome resources provide unparalleled opportunities for investigating such unusual mechanisms of cellulose conversion. More broadly, the genome offers insight into the diversification of lignocellulose degrading mechanisms in fungi. Comparisons with the closely related white-rot fungus Phanerochaete chrysosporium support an evolutionary shift from white-rot to brown-rot during which the capacity for efficient depolymerization of lignin was lost. This work was supported by the U.S. Department of Energy’s Office of Science, Biological and Environmental Research Program, and University of California, Lawrence Berkeley National Laboratory Contract DE-AC02–05CH11231; Lawrence Livermore National Laboratory Contract DE-AC52–07NA27344; Los Alamos National Laboratory Contract DE-AC02–06NA25396; University of Wisconsin Grant DE-FG02–87ER13712; Forest Products Laboratory, U.S. Department of Agriculture, Cooperative State Research, Education, and Extension Services Grant 2007–35504-18257; National Institutes of Health Grant GM060201 (to University of New Mexico); Centro de Investigaciones Biológicas (Madrid) EUproject NMP2–2006-026456; Ministry of Education Czech Republic Grant LC06066. |
| Databáze: | OpenAIRE |
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