Comprehensive characterization of the functional activities of pressurized liquid and ultrasound-assisted extracts from Chlorella vulgaris
| Autor: | Susana Santoyo, Merichel Plaza, Laura Jaime, Elena Ibáñez, Francisco J. Señoráns, Guillermo Reglero, Belkis Avalo, Alejandro Cifuentes, Guillermo Garcia-Blairsy Reina |
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| Přispěvatelé: | Ministerio de Educación y Ciencia (España), Comunidad de Madrid, Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | et al. Chlorella vulgaris has been referred as a potential source of bioactive compounds (carotenoids and fatty acids). In this work, the ability of an environmentally friendly extraction technique such as Pressurized Liquid Extraction (PLE) and a traditional extraction technique such as Ultrasound-Assisted Extraction (UAE) to obtain functional compounds from C. vulgaris, at analytical scale, has been demonstrated. Seeked bioactivities were antioxidant and antimicrobial, for their interest in the food industry. Therefore, a methodology including analytical extraction, in-vitro assays and chemical characterization via HPLC-DAD and GC-MS has been used to determine the interest of Chlorella as a source of functional food ingredients. Results demonstrated that PLE provide higher yields than UAE while similar bioactivities were obtained. Important concentration of carotenoids (lutein, neoxanthin, β-carotene, etc.), chlorophylls, sterols, phytols, and fatty acids (among others) have been found in Chlorella extracts that could be correlated to the observed biological activity. © 2011 Elsevier Ltd. This work has been financed by Spanish Ministry of Education and Science (AGL2005-06726-C04-01/02/04 and CONSOLIDER INGENIO 2010 CSD2007-00063 FUN-C-FOOD) and Comunidad Autónoma de Madrid (S-0505/AGR/000153) projects. M.P. thanks CSIC for her I3P fellowship. |
| Databáze: | OpenAIRE |
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