Isolation and transmission of the Lyme disease spirochete from the southeastern United States
| Autor: | B S McGuire, D. E. Stallknecht, M. P. Luttrell, Robert S. Lane, J. H. Oliver, A. M. James, F. W. Chandler, G. A. Cummins, H. J. Hutcheson |
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| Rok vydání: | 1993 |
| Předmět: |
DNA
Bacterial Georgia Lipoproteins Molecular Sequence Data Animals Wild Spirochaetaceae Tick Polymerase Chain Reaction Microbiology Amblyomma americanum Lyme disease Peromyscus Ticks Borrelia burgdorferi Group parasitic diseases medicine Animals Borrelia burgdorferi Lyme Disease Multidisciplinary biology Base Sequence biology.organism_classification medicine.disease Ixodes affinis Virology Bacterial vaccine Oligodeoxyribonucleotides Ixodes scapularis Antigens Surface Bacterial Vaccines Arachnid Vectors Bacterial Outer Membrane Proteins Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 90(15) |
| ISSN: | 0027-8424 |
| Popis: | The isolation of the Lyme disease spirochete (Borrelia burgdorferi) from the southeastern United States is reported. Three isolates, two from cotton mice (Peromyscus gossypinus) and one from the black-legged tick (Ixodes scapularis), were recovered from Sapelo Island, Georgia, in July and September 1991. The spirochetes were characterized by indirect fluorescent antibody assay using a battery of five monoclonal antibodies, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE) of whole cell lysates, and by the polymerase chain reaction (PCR) assay using primers for three DNA target sequences found in B. burgdorferi reference strain B-31. Transmission experiments indicate that the three Georgia isolates can infect experimentally inoculated hamsters and mice. Tick transmission of one of the isolates has been attempted so far; I. scapularis transmitted isolate SI-1 from hamsters to mice, but the lone-star tick, Amblyomma americanum, did not. |
| Databáze: | OpenAIRE |
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