Application of CPI cutoff value based on parentage testing of duos and trios typed by four autosomal kits

Autor: Hanyang Wu, Ruxia Zhang, Shanhui Sun, Maoxiu Zhang, Hong-Mei Gao, Dongjie Xiao, Chang Wang, Yunshan Wang
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Forensic Genetics
Male
Value (computer science)
Social Sciences
Pentas
Paternity
Geographical Locations
0302 clinical medicine
Statistics
Medicine and Health Sciences
Cutoff
DNA extraction
Flowering Plants
Mathematics
Multidisciplinary
Sex Chromosomes
Chromosome Biology
Eukaryota
X Chromosomes
Genomics
Plants
Y Chromosomes
Clinical Laboratory Sciences
Tandem Repeats
Medicine
Female
Research Article
China
Asia
Paternity Index
Science
Chromosomes
03 medical and health sciences
Extraction techniques
Diagnostic Medicine
Genetics
Humans
030216 legal & forensic medicine
Repeated Sequences
Forensics
Organisms
Biology and Life Sciences
Cell Biology
Research and analysis methods
030104 developmental biology
Genetic Loci
People and Places
Law and Legal Sciences
Microsatellite Repeats
Zdroj: PLoS ONE, Vol 14, Iss 11, p e0225174 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: In this study, we analyzed the application of four autosomal kits and the sensitivity of the combined paternity index (CPI) cutoff value (CPI≥10000) in parentage testing. First, 1442 real trios and 803 real duos were tested using the Goldeneye 25A kit. The Goldeneye 25A kit covers the autosomal short tandem repeat (STR) loci of the other three kits, so we calculated the CPI value of every case for the four kits. Second, three complex close relative kinship cases were also analyzed to evaluate the application of the CPI cutoff value. The CPI values of all trio cases were higher than 10000 using the four kits; the CPI values of all duo cases were higher than 10000 using the Goldeneye 25A kit; and the CPI values of a portion of the duo cases were lower than 10000 using the other three kits. In the three complex close relative cases, the alleged father or mother was not excluded using 40 autosomal STRs. Adding X chromosome short tandem repeats (X-STR) and samples of biological fathers or mothers, the conclusions were confirmed. The four kits were adequate to draw conclusions in the trio cases; the Goldeneye 25A Kit was adequate to draw conclusions in the duo cases; and the other three kits were not sufficient for a portion of the duo cases. The CPI cutoff value was sensitive for real trio and duo cases. In complex close relative kinship cases, high CPI values may result in false conclusions.
Databáze: OpenAIRE
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