ARABIDILLO proteins have a novel and conserved domain structure important for the regulation of their stability
| Autor: | Karen A. Bunting, Laura A. Moody, Susan J. Bradshaw, Juliet C. Coates, Emma J. Smiles, Cândida Nibau, Jennifer A. Tubby, Daniel J. Gibbs |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Repetitive Sequences Amino Acid Proteasome Endopeptidase Complex Blotting Western Molecular Sequence Data Protein domain Plant Science Leucine-rich repeat F-box protein Gene Expression Regulation Plant Two-Hybrid System Techniques Arabidopsis Genetics Amino Acid Sequence Cell Cycle Protein Conserved Sequence Phylogeny beta Catenin Binding Sites SKP Cullin F-Box Protein Ligases Sequence Homology Amino Acid biology Arabidopsis Proteins Protein Stability Reverse Transcriptase Polymerase Chain Reaction Ubiquitin F-Box Proteins General Medicine biology.organism_classification Protein Structure Tertiary Proteasome Biochemistry Armadillo repeats Mutation biology.protein Agronomy and Crop Science Function (biology) Protein Binding |
| Zdroj: | Plant Molecular Biology. 75:77-92 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/s11103-010-9709-1 |
| Popis: | ARABIDILLO proteins are F-box-Armadillo (ARM) proteins that regulate root branching in Arabidopsis. Many F-box proteins in plants, yeast and mammals are unstable. In plants, the mechanism for this instability has not been fully investigated. Here, we show that a conserved family of plant ARABIDILLO-related proteins has a unique domain structure consisting of an F-box and leucine-rich repeats (LRRs) followed by ARM-repeats. The LRRs are similar to those found in other plant and animal F-box proteins, including cell cycle proteins and hormone receptors. We demonstrate that the LRRs are required for ARABIDILLO1 function in vivo. ARABIDILLO1 protein is unstable: we show that ARABIDILLO1 protein is associated with ubiquitin and is turned over by the proteasome. Both the F-box and LRR regions of ARABIDILLO1 appear to enable this turnover to occur. Application of known lateral root-regulating signals has no effect on ARABIDILLO1 stability. In addition, plants that lack or overexpress ARABIDILLO proteins respond normally to known lateral root-regulating signals. Thus, we suggest that the signal(s) regulating ARABIDILLO stability in vivo may be either highly specific or novel. The structural conservation between ARABIDILLOs and other plant and animal F-box proteins suggests that the stability of other F-box proteins may be controlled by similar mechanisms. |
| Databáze: | OpenAIRE |
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