miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study
| Autor: | Francisco Alpiste-Illueca, Marta Seco-Cervera, Andrés López-Roldán, Pablo Micó-Martínez, J. L. García-Giménez, Pedro J Almiñana-Pastor, Federico V. Pallardó |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology Adult Male medicine.medical_specialty Pilot Projects Disease 03 medical and health sciences Internal medicine microRNA medicine Humans General Dentistry Periodontitis Regulation of gene expression Messenger RNA Oral Medicine and Pathology business.industry Research Gingival Crevicular Fluid Middle Aged CIENCIAS MÉDICAS [UNESCO] medicine.disease Chronic periodontitis Reverse transcription polymerase chain reaction MicroRNAs 030104 developmental biology Otorhinolaryngology Chronic Periodontitis UNESCO::CIENCIAS MÉDICAS Biomarker (medicine) Surgery Female business Biomarkers |
| Zdroj: | Micó Martínez, Pablo ; Garcia Gimenez, Jose Luis ; Seco Cervera, Marta ; López Roldán, Andrés ; Almiñana Pastor, Pedro J. ; Alpiste Illueca, Francisco M. ; Pallardó Calatayud, Federico V. miR-1226 detection in GCF as potential biomarker of chronic periodontitis: a pilot study. En: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, 23 3 2018: 8 Medicina Oral, Patología Oral y Cirugía Bucal RODERIC. Repositorio Institucional de la Universitat de Valéncia instname |
| Popis: | Background The study and identification of new biomarkers for periodontal disease, such as microRNAs (miRNAs), may give us more information about the location and severity of the disease and will serve as a basis for treatment planning and disease-monitoring. miRNAs are a group of small RNAs which are involved in gene regulation by binding to their messenger RNA target (mRNA). In this pilot study, the procedure for purifying miRNAs from gingival crevicular fluid (GCF) was, for the first time, described. In addition, the concentration of miRNAs in GCF was analyzed and compared between patients with moderate or severe chronic periodontitis (CP) and healthy controls. Material and Methods GCF samples were collected from single-rooted teeth of patients with moderate or severe CP (n=9) and of healthy individuals (n=9). miRNAs were isolated from GCF using miRNeasy Serum/Plasma kit (Qiagen, CA. USA). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression of a series of miRNAs candidates that are related to bone metabolism. The significance of differences in miRNA levels between both groups was determined using Mann-Whitney U test. Results The results from this pilot study indicate that miRNAs can be isolated from GCF. Six different miRNAs were analyzed (miR-671, miR-122, miR-1306, miR-27a, miR-223, miR-1226), but only miR-1226 showed statically significant differences between the CP group and healthy controls (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|